CyFlow™ CD32 Purified

CyFlow™ CD32 Purified
Target Species: Human
Antigen: CD32
Field of Interest: Immunophenotyping
Clone: 3D3
Regulatory Status: RUO
Alternative Name: FcγRII
Application: Flow cytometry
Format/Fluorochrome: Purified
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: BP575450

For Research Use Only

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Immunogen Purified... more
CyFlow™ CD32 Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenPurified glycosylated recombinant human FcgammaRIIa2
SpecifityThe mouse monoclonal antibody 3D3 recognizes CD32 antigen, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant).
Background InformationCD32 (FcγRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes).
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Vely F, Gruel N, Moncuit J, Cochet O, Rouard H, Dare S, Galon J, Sautes C, Fridman WH, Teillaud JL: A new set of monoclonal antibodies against human Fc gamma RII (CD32) and Fc gamma RIII (CD16): characterization and use in various assays. Hybridoma. 1997·Dec; 16(6):519‑28. <·PMID:·9455704·> | Boruchov AM, Heller G, Veri MC, Bonvini E, Ravetch JV, Young JW: Activating and inhibitory IgG Fc receptors on human DCs mediate opposing functions. J·Clin·Invest. 2005·Oct; 115(10):2914‑23. <·PMID:·16167082·> | Dutertre CA, Bonnin-Gélizé E, Pulford K, Bourel D, Fridman WH, Teillaud JL: A novel subset of NK cells expressing high levels of inhibitory FcgammaRIIB modulating antibody‑dependent function. J·Leukoc·Biol. 2008·Dec; 84(6):1511‑20. <·PMID:·18719017·> | Dai X, Jayapal M, Tay HK, Reghunathan R, Lin G, Too CT, Lim YT, Chan SH, Kemeny DM, Floto RA, Smith KG, Melendez AJ, MacAry PA: Differential signal transduction, membrane trafficking, and immune effector functions mediated by FcgammaRI versus FcgammaRIIa. Blood. 2009·Jul·9; 114(2):318‑27. <·PMID:·19420354·> | Os A, Bürgler S, Ribes AP, Funderud A, Wang D, Thompson KM, Tjønnfjord GE, Bogen B, Munthe LA: Chronic lymphocytic leukemia cells are activated and proliferate in response to specific T helper cells. Cell·Rep. 2013·Aug·15; 4(3):566‑77. <·PMID:·23933259·>

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