CyFlow™ CD22 Purified

CyFlow™ CD22 Purified
Target Species: Human, Non-Human Primates
Antigen: CD22
Field of Interest: Immunophenotyping
Clone: MEM-01
Regulatory Status: RUO
Alternative Name: BL-CAM, SIGLEC-2, SIGLEC2
Application: Flow cytometry, Immunoprecipitation, Western blot
Format/Fluorochrome: Purified
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: BH159259

For Research Use Only

$120.00 USD*

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Immunogen Raji cells:... more
CyFlow™ CD22 Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenRaji cells: human Burkitt's lymphoma cell line
SpecifityThe mouse monoclonal antibody MEM-01 recognizes CD22 antigen, a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E"; it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.
Background InformationCD22 (Siglec-2; sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding α 2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Tedder TF, Poe JC, Haas KM: CD22: A Multifunctional Receptor That Regulates B Lymphocyte Survival and Signal Transduction. Adv·Immunol. 2005; 88:1‑50. <·PMID:·16227086·> | Tateno H, Li H, Schur MJ, Bovin N, Crocker PR, Wakarchuk WW, Paulson JC: Distinct endocytic mechanisms of CD22 (Siglec‑2 and Siglec‑F reflect roles in cell signaling and innate immunity. Mol·Cell·Biol. 2007·Aug; 27(16):5699‑710. <·PMID:·17562860·> | Walker JA, Smith KG: CD22: An inhibitory enigma. Immunology. 2008·Mar; 123(3):314‑25. <·PMID:·18067554·>

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