CyFlow™ CD21 Purified

CyFlow™ CD21 Purified
Target Species: Cow, Dog, Human, Pig
Antigen: CD21
Field of Interest: Immunophenotyping
Clone: LT21
Regulatory Status: RUO
Alternative Name: C3dR, CR2, EBV-R
Application: Flow cytometry, Immunohistochemistry (frozen sections)
Format/Fluorochrome: Purified
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: BJ686541

For Research Use Only

$120.00 USD*

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HLDA Workshop HLDA VI—WS Code B CD21.1 Concentration 1 mg/mL Quantity 0.1 mg... more
CyFlow™ CD21 Purified
HLDA WorkshopHLDA VI—WS Code B CD21.1
Concentration1 mg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenIM9 human B-lymphoblastoid cell line
Background InformationCD21 (CR2; complement receptor 2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Kasprzak A, Spachacz R, Wachowiak J, Stefanska K, Zabel M: Epstein‑Barr virus (EBV) infection in B‑cell non‑Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules. Folia·Histochem·Cytobiol. 2007; 45(3):169‑79. <·PMID:·17951165·> | Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass‑spectrometry. J·Immunol·Methods. 2007·Jan·30; 319(1‑2):21‑33. <·PMID:·17098248·> | Twohig J, Kulik L, Haluszczak C, Reuter J, Rossbach A, Bull M, Holers VM, Marchbank KJ: Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21 transgenic mice is partially recovered in the absence of C3. Mol·Immunol. 2007·Jul; 44(13):3434‑44. <·PMID:·17379312·> | Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross‑reactive anti‑human monoclonal antibodies as a tool for B‑cell identification in dogs and pigs. Vet·Immunol·Immunopathol. 2007·Sep·15; 119(1‑2):56‑62. <·PMID:·17673300·> | Roozendaal R, Carroll MC: Complement receptors CD21 and CD35 in humoral immunity. Immunol·Rev. 2007·Oct; 219:157‑66. <·PMID:·17850488·> | Zabel MD, Heikenwalder M, Prinz M, Arrighi I, Schwarz P, Kranich J, von Teichman A, Haas KM, Zeller N, Tedder TF, Weis JH, Aguzzi A: Stromal complement receptor CD21/35 facilitates lymphoid prion colonization and pathogenesis. J·Immunol. 2007·Nov·1; 179(9):6144‑52. <·PMID:·17947689·> | Singh A, Blank M, Shoenfeld Y, Illges H: Antiphospholipid syndrome patients display reduced titers of soluble CD21 in their sera irrespective of circulating anti‑beta2‑glycoprotein‑I autoantibodies. Rheumatol·Int. 2008·Jan·3; <·PMID:·23949624·>

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