CyFlow™ CD19 Biotin

CyFlow™ CD19 Biotin
Target Species: Human
Antigen: CD19
Field of Interest: Immunophenotyping
Clone: 4G7
Regulatory Status: RUO
Alternative Name: B4
Application: Flow cytometry
Format/Fluorochrome: Biotin
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: CG759445

For Research Use Only

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Immunogen Human CCL... more
CyFlow™ CD19 Biotin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenHuman CCL (chronic lymphocytic leukemia) cells
SpecifityThe mouse monoclonal antibody 4G7 recognizes CD19 antigen, a 95 kDa type I transmembrane glycoprotein of immunoglobulin superfamily, expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
Background InformationCD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

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