CyFlow™ CD11b Azide Free

CyFlow™ CD11b Azide Free
Target Species: Human
Antigen: CD11b
Field of Interest: Immunophenotyping
Clone: MEM-174
Regulatory Status: RUO
Alternative Name: Mac-1
Application: Flow cytometry, Immunoprecipitation
Format/Fluorochrome: Azide Free
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG2a
Product number: AT384109

For Research Use Only

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Immunogen Human... more
CyFlow™ CD11b Azide Free
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
ImmunogenHuman granulocytes
SpecifityThe mouse monoclonal antibody MEM-174 recognizes CD11b antigen, a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
Background InformationCD11b (integrin αM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with CD18 (integrin β2 subunit); expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI: Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule‑1. Blood. 2000·Feb·1; 95(3):911‑20. <·PMID:·10648403·> | Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E: Effects of beta‑glucans on the immune system. Medicina·(Kaunas). 2007; 43(8):597‑606. <·PMID:·17895634·> | Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·> | Lawrence PK, Srikumaran S: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization. Vet·Immunol·Immunopathol. 2007·Oct·15; 119(3‑4):287‑98. <·PMID:·17628696·>

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