CyFlow™ CD9 APC-Cy7

CyFlow™ CD9 APC-Cy7
Laser: Red
Target Species: Human
Antigen: CD9
Field of Interest: Immunophenotyping
Clone: MEM-61
Regulatory Status: RUO
Alternative Name: MRP-1, P24
Application: Flow cytometry
Format/Fluorochrome: APC-Cy7
Species of Origin: Mouse
Clonality: monoclonal
Emission Maximum: 780 nm
Excitation Maximum: 650 nm, 750 nm
Isotype: IgG1
Product number: CD441220

For Research Use Only

$295.00 USD*

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HLDA Workshop HLDA VI—WS Code P P-15 Concentration Quantity 100 tests Volume... more
CyFlow™ CD9 APC-Cy7
HLDA WorkshopHLDA VI—WS Code P P-15
Concentration
Quantity100 tests
Volume0.4 mL
ImmunogenNALM-6 human pre-B cell line
Background InformationCD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA‑A1‑restricted, hapten‑specific CTL. J·Immunol. 2001·Sep·1; 167(5):2724‑33. <·PMID:·11509616·> | Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I: Absence of CD9 enhances adhesion‑dependent morphologic differentiation, survival, and matrix metalloproteinase‑2 production in small cell lung cancer cells. Cancer·Res. 2006·Oct·1; 66(19):9557‑65. <·PMID:·17018612·> | Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc·Natl·Acad·Sci·USA. 2007·Jan·2; 104(1):234‑9. <·PMID:·17190803·> | Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb·Haemost. 2007·Nov; 98(5):1081‑7. <·PMID:·18000614·> | Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose‑derived mesenchymal stem cells. Pflugers·Arch. 2007·Nov; 455(2):283‑96. <·PMID:·17668233·> | Singh AB, Sugimoto K, Dhawan P, Harris RC: Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am·J·Physiol·Cell·Physiol. 2007·Nov; 293(5):C1660‑8. <·PMID:·17855771·> | Lafleur MA, Xu D, Hemler ME: Tetraspanin proteins regulate membrane type‑1 matrix metalloproteinase‑dependent pericellular proteolysis. Mol·Biol·Cell. 2009·Apr; 20(7):2030‑40. <·PMID:·19211836·>

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