CyFlow™ CD5 Purified

CyFlow™ CD5 Purified
Target Species: Human
Antigen: CD5
Field of Interest: Immunophenotyping
Clone: MEM-32
Regulatory Status: RUO
Alternative Name: Leu-1, T1
Application: Enzyme-linked immunosorbent assay, Flow cytometry, Immunohistochmistry (paraffin-embedded sections), Immunoprecipitation, Western blot
Format/Fluorochrome: Purified
Species of Origin: Mouse
Clonality: monoclonal
Isotype: IgG1
Product number: CZ785326

For Research Use Only

$120.00 USD*

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HLDA Workshop HLDA III—WS Code T 523 Concentration 1 mg/mL Quantity 0.1 mg... more
CyFlow™ CD5 Purified
HLDA WorkshopHLDA III—WS Code T 523
Concentration1 mg/mL
Quantity0.1 mg
Volume0.1 mL
ImmunogenCrude thymus membrane fraction
Background InformationCD5 (T1) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens (I); Antibodies against beta‑2‑microglobulin, immunoglobulin kappa light chains, HLA‑DR‑like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan‑leucocyte antigen. Folia·Biol·(Praha). 1986; 32(1):12‑25. <·PMID:·2422063·> | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·> | Freedman AS, Freeman G, Whitman J, Segil J, Daley J, Levine H, Nadler LM: Expression and regulation of CD5 on in vitro activated human B cells. Eur·J·Immunol. 1989·May; 19(5):849‑55. <·PMID:·2472277·> | Raman C: CD5, an important regulator of lymphocyte selection and immune tolerance. Immunol·Res. 2002; 26(1‑3):255‑63. <·PMID:·12403363·> | Hrdinka M, Dráber P, Stepánek O, Ormsby T, Otáhal P, Angelisová P, Brdicka T, Paces J, Horejsí V, Drbal K: PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis. J·Biol·Chem. 2011·Jun·3; 286(22):19617‑29. <·PMID:·21460222·>

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