CyFlow™ CD117 PE-Cy7
Alternative Name: | c-kit , SCFR |
Antibody: | Yes |
Antigen: | CD117 |
Clonality: | monoclonal |
Clone: | 104D2 |
Emission Maximum: | 785 nm |
Excitation Maximum: | 496 nm, 565 nm |
Field of Interest: | Immunophenotyping, Phosphorylation |
Format/Fluorochrome: | PE-Cy7 |
Isotype: | IgG1 |
Laser: | Blue , Green, Yellow |
Regulatory Status: | ASR |
Source Species: | Mouse |
Target Species: | Human |
Product number: | CF030503 |
Analytical and performance characteristics are not established
HLDA Workshop | HLDA VI—WS Code C-30 |
Concentration Unit | µg/mL |
Concentration | 50 |
Volume | 1.0 mL |
Immunogen | MOLM-1 megakaryocytic cells |
Antigen Distribution | CD117 (c-kit, SCFR) is a 145 kDa receptor tyrosine kinase expressed on pluripotent hematopoietic progenitor cells and mast cells. It regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. After binding of its ligand, SCF (stem cell factor), CD117 is autophosphorylated on its intracellular domains and activated. |
Usage | The reagent may have application in various test methodologies, including Flow Cytometry. Vial has sufficient volume for 100 tests, if used at 10 μL·/·test. User has responsibility for determining reagent quantity per test for any ASR reagent. |
Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997·Jul·1; 90(1):111‑25. <·PMID:·9207445·> | Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c‑kit receptor dimerization by fluorescence resonance energy transfer. Blood. 1998·Feb·1; 91(3):898‑906. <·PMID:·9446650·> | Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c‑Kit. Blood. 1999·Sep·15; 94(6):1979‑86. <·PMID:·10477727·> | Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti‑human cross‑reactive antibodies. Exp·Anim. 2000·Apr; 49(2):97‑110. <·PMID:·10889948·> | Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long‑term proliferative ability in vitro and in vivo lack surface expression of c‑kit (CD117). Exp·Hematol. 2000·Jun; 28(6):660‑71. <·PMID:·10880752·> | Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk·Res. 2006·Oct; 30(10):1241‑8. <·PMID:·16387359·> | Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007·Jul·1; 110(1):151‑60. <·PMID:·17379743·> | Stevenson KS, McGlynn L, Hodge M, McLinden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem·Cells·Dev. 2009·Dec; 18(10):1389‑98. <·PMID:·19326970·>
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