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CyFlow™ CD117 PE-Cy7

CyFlow™ CD117 PE-Cy7
Alternative Name: c-kit , SCFR
Antibody: Yes
Antigen: CD117
Clonality: monoclonal
Clone: 104D2
Emission Maximum: 785 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping, Phosphorylation
Format/Fluorochrome: PE-Cy7
Isotype: IgG1
Laser: Blue , Green, Yellow
Regulatory Status: ASR
Source Species: Mouse
Target Species: Human
Product number: CF030503

Analytical and performance characteristics are not established

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HLDA Workshop HLDA VI—WS Code C-30 Concentration Unit µg/mL Concentration 50... more
CyFlow™ CD117 PE-Cy7

HLDA WorkshopHLDA VI—WS Code C-30
Concentration Unitµg/mL
Concentration50
Volume1.0 mL
ImmunogenMOLM-1 megakaryocytic cells
Antigen DistributionCD117 (c-kit, SCFR) is a 145 kDa receptor tyrosine kinase expressed on pluripotent hematopoietic progenitor cells and mast cells. It regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. After binding of its ligand, SCF (stem cell factor), CD117 is autophosphorylated on its intracellular domains and activated.
UsageThe reagent may have application in various test methodologies, including Flow Cytometry. Vial has sufficient volume for 100 tests, if used at 10 μL·/·test. User has responsibility for determining reagent quantity per test for any ASR reagent.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997·Jul·1; 90(1):111‑25. <·PMID:·9207445·> | Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c‑kit receptor dimerization by fluorescence resonance energy transfer. Blood. 1998·Feb·1; 91(3):898‑906. <·PMID:·9446650·> | Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c‑Kit. Blood. 1999·Sep·15; 94(6):1979‑86. <·PMID:·10477727·> | Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti‑human cross‑reactive antibodies. Exp·Anim. 2000·Apr; 49(2):97‑110. <·PMID:·10889948·> | Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long‑term proliferative ability in vitro and in vivo lack surface expression of c‑kit (CD117). Exp·Hematol. 2000·Jun; 28(6):660‑71. <·PMID:·10880752·> | Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk·Res. 2006·Oct; 30(10):1241‑8. <·PMID:·16387359·> | Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007·Jul·1; 110(1):151‑60. <·PMID:·17379743·> | Stevenson KS, McGlynn L, Hodge M, McLinden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem·Cells·Dev. 2009·Dec; 18(10):1389‑98. <·PMID:·19326970·>

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