CyFlow™ CD45 APC-Cy7
| Alternative Name: | B220, LCA , T200 |
| Antibody: | Yes |
| Antigen: | CD45 |
| Clonality: | monoclonal |
| Clone: | 2D1 |
| Emission Maximum: | 780 nm |
| Excitation Maximum: | 650 nm, 750 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | APC-Cy7 |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | ASR |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CU144358 |
Analytical and performance characteristics are not established
| HLDA Workshop | HLDA III – WS Code 792 |
| Concentration Unit | µg/mL |
| Concentration | 65 |
| Volume | 1.0 mL |
| Immunogen | Human peripheral blood mononuclear cells (PBMC) |
| Antigen Distribution | CD45 (LCA, T200, B220) is a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. |
| Usage | The reagent may have application in various test methodologies, including Flow Cytometry. Vial has sufficient volume for 100 tests, if used at 10 μL·/·test. User has responsibility for determining reagent quantity per test for any ASR reagent. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·> | Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens (II); Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18‑kDa antigen (MEM‑43) and a novel antigen of restricted expression (MEM‑74). Folia·Biol·(Praha). 1988; 34(1):23‑34. <·PMID:·2968928·> | Koene HR, Kleijer M, Algra J, Roos D, von dem Borne AE, de Haas M: Fc gammaRIIIa‑158V/F polymorphism influences the binding of IgG by natural killer cell Fc gammaRIIIa, independently of the Fc gammaRIIIa‑48L/R/H phenotype. Blood. 1997·Aug·1; 90(3):1109‑14. <·PMID:·9242542·> | Bhunia AK, Arai T, Bulkley G, Chatterjee S: Lactosylceramide mediates tumor necrosis factor‑alpha‑induced intercellular adhesion molecule‑1 (ICAM‑1 expression and the adhesion of neutrophil in human umbilical vein endothelial cells. J·Biol·Chem. 1998·Dec·18; 273(51):34349‑57. <·PMID:·9852101·> | Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J·Biol·Chem. 2002·Mar·8; 277(10):7955‑61. <·PMID:·11773067·> | Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J·Immunol. 2006·Mar·15; 176(6):3417‑25. <·PMID:·16517710·> | Cortez-Gonzalez X, Sidney J, Adotevi O, Sette A, Millard F, Lemonnier F, Langlade-Demoyen P, Zanetti M: Immunogenic HLA‑B7‑restricted peptides of hTRT. Int·Immunol. 2006·Dec; 18(12):1707‑18. <·PMID:·17077179·> | Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H: Role for CD47‑SIRPalpha signaling in xenograft rejection by macrophages. Proc·Natl·Acad·Sci·USA. 2007·Mar·20; 104(12):5062‑6. <·PMID:·17360380·> | Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·> | Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem·J. 2007·Sep·1; 406(2):333‑41. <·PMID:·17497959·> | Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008·Feb; 13(2):197‑212. <·PMID:·18157742·> | McCormack E, Mujić M, Osdal T, Bruserud Ø , Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time‑domain imaging of acute myeloid leukemia. Blood. 2013·Feb·14; 121(7):e34‑42. <·PMID:·23243270·> | McCormack E, Mujić M, Osdal T, Bruserud Ø , Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time‑domain imaging of acute myeloid leukemia. Blood. 2013·Feb·14; 121(7):e34‑42. <·PMID:·23243270·>
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