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CyFlow™ CD5 PE-Cy7

CyFlow™ CD5 PE-Cy7
Alternative Name: Leu-1, T1
Antibody: Yes
Antigen: CD5
Clonality: monoclonal
Clone: L17F12
Emission Maximum: 785 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE-Cy7
Isotype: IgG2a
Laser: Blue , Green, Yellow
Regulatory Status: ASR
Source Species: Mouse
Target Species: Human
Product number: BZ394937

Analytical and performance characteristics are not established

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HLDA Workshop HLDA II Concentration Unit µg/mL Concentration 20 Volume 1.0 mL... more
CyFlow™ CD5 PE-Cy7

HLDA WorkshopHLDA II
Concentration Unitµg/mL
Concentration20
Volume1.0 mL
ImmunogenHuman Acute Lymphocytic Leukemia (ALL) T cells
Antigen DistributionCD5 (T1, Leu-1) is a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes). It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.
UsageThe reagent may have application in various test methodologies, including Flow Cytometry. Vial has sufficient volume for 100 tests, if used at 10 μL·/·test. User has responsibility for determining reagent quantity per test for any ASR reagent.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R: Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc·Natl·Acad·Sci·USA. 1981·Mar; 78(3):1791‑5. <·PMID:·7015346·> | Shuster JJ, Falletta JM, Pullen DJ, Crist WM, Humphrey GB, Dowell BL, Wharam MD, Borowitz M: Prognostic factors in childhood T‑cell acute lymphoblastic leukemia: a Pediatric Oncology Group study. Blood. 1990·Jan·1; 75(1):166‑73. <·PMID:·1688495·> | McAlister MS, Davis B, Pfuhl M, Driscoll PC: NMR analysis of the N‑terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. Protein·Eng. 1998·Oct; 11(10):847‑53. <·PMID:·9862202·> | Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ: Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am·J·Clin·Pathol. 2001·Dec; 116(6):893‑7. <·PMID:·11764079·> | Dunphy CH, Tang W: The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases. Arch·Pathol·Lab·Med. 2007·May; 131(5):748‑54. <·PMID:·17488160·>

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