CyFlow™ CD3 APC-Cy7
| Alternative Name: | Leu4, T3 |
| Antibody: | Yes |
| Antigen: | CD3 |
| Clonality: | monoclonal |
| Clone: | SK7 |
| Emission Maximum: | 780 nm |
| Excitation Maximum: | 650 nm, 750 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | APC-Cy7 |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | ASR |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AX394879 |
Analytical and performance characteristics are not established
| HLDA Workshop | HLDA II—WS Code T118 HLDA III—WS Code T492 |
| Concentration Unit | µg/mL |
| Concentration | 25 |
| Volume | 1.0 mL |
| Immunogen | Human thymocytes |
| Antigen Distribution | CD3 (T3, Leu4) is expressed on peripheral blood lymphocytes, thymocytes and Purkinje cells in the cerebellum; it is lost on B or NK cells. CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3γ, CD3δ, CD3ε and CD3ζ. |
| Usage | The reagent may have application in various test methodologies, including Flow Cytometry. Vial has sufficient volume for 100 tests, if used at 10 μL·/·test. User has responsibility for determining reagent quantity per test for any ASR reagent. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Garson JA, Beverley PC, Coakham HB, Harper EI: Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982·Jul·22; 298(5872):375‑7. <·PMID:·6178042·> | McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·> | Fisch P, Malkovsky M, Braakman E, Sturm E, Bolhuis RL, Prieve A, Sosman JA, Lam VA, Sondel PM: Gamma/delta T cell clones and natural killer cell clones mediate distinctpatterns of non‑major histocompatibility complex‑restricted cytolysis. J·Exp·Med. 1990·May·1; 171(5):1567‑79. <·PMID:·2185329·> | Barclay, Brown, et al.: The Leukocyte Antigen FactsBook; 2nd edition. Harcourt·Brace·&·Company,·London. 1997; <·No·PMID·> | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | le Gouvello S, Manceau V, Sobel A: Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin‑dependent kinase II after CD2 triggering of human T lymphocytes. J·Immunol. 1998·Aug·1; 161(3):1113‑22. <·PMID:·9686569·> | Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J·Immunol. 2003·Jun·15; 170(12):5947‑55. <·PMID:·12794121·> | Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J·Biol·Chem. 2004·Jul·9; 279(28):28827‑3. <·PMID:·15084594·> | Arnett KL, Harrison SC, Wiley DC: Crystal structure of a human CD3‑epsilon/delta dimer in complex with a UCHT1 single‑chain antibody fragment. Proc·Natl·Acad·Sci·USA. 2004·Nov·16; 101(46):16268‑73. <·PMID:·15534202·> | Demedts IK, Brusselle GG, Vermaelen KY, Pauwels RA: Identification and characterization of human pulmonary dendritic cells. Am·J·Respir·Cell·Mol·Biol. 2005·Mar; 32(3):177‑84. <·PMID:·15576669·> | Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005·Nov·15; 106(10):3567‑74. <·PMID:·16046525·> | Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006·Feb; 24(2):133‑9. <·PMID:·16473826·> | Alarcón B, Swamy M, van Santen HM, Schamel WW: T‑cell antigen‑receptor stoichiometry: pre‑clustering for sensitivity. EMBO·Rep. 2006·May; 7(5):490‑5. <·PMID:·16670682·> | Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T‑cell deficiency. N·Engl·J·Med. 2006·May·4; 354(18):1913‑21. <·PMID:·16672702·> | Siegers GM, Swamy M, Fernández-Malavé E, Minguet S, Rathmann S, Guardo AC, Pérez-Flores V, Regueiro JR, Alarcón B, Fisch P, Schamel WW: Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J·Exp·Med. 2007·Oct·29; 204(11):2537‑44. <·PMID:·17923503·>
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