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CyFlow™ CD20 Pacific Blue™

CyFlow™ CD20 Pacific Blue™
Alternative Name: B1 , Bp35
Antibody: Yes
Antigen: CD20
Clonality: monoclonal
Clone: 2H7
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Field of Interest: Immunophenotyping, Signaling
Format/Fluorochrome: Pacific Blue™
Isotype: IgG2b
Laser: Violet
Regulatory Status: ASR
Source Species: Mouse
Target Species: Human
Product number: AQ315269

Analytical and performance characteristics are not established

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HLDA Workshop HLDA IV—WS Code B201 Concentration Unit µg/mL Concentration 100... more
CyFlow™ CD20 Pacific Blue™

HLDA WorkshopHLDA IV—WS Code B201
Concentration Unitµg/mL
Concentration100
Volume1.0 mL
ImmunogenHuman tonsillar B cells
Antigen DistributionCD20 (B1, Bp35) is a cell surface 33-37 kDa (depending on the degree of phosphorylation) non-glycosylated surface phosphoprotein expressed on B cells; it is lost on stem cells and plasma cells. Low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells. Its expression on B cells is synchronous with the expression of surface IgM.
UsageThe reagent may have application in various test methodologies, including Flow Cytometry. Vial has sufficient volume for 100 tests, if used at 10 μL·/·test. User has responsibility for determining reagent quantity per test for any ASR reagent.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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