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CyFlow™ Lambda-LC FITC

CyFlow™ Lambda-LC FITC
Antibody: Yes
Antigen: λ light chains
Application: Flow cytometry
Clonality: monoclonal
Clone: 4C2
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunoglobulins
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BZ611999

For Research Use Only

$235.00 USD*

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 2.0 mL Immunogen Not available Background Information... more
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CyFlow™ Lambda-LC FITC

Quantity100 tests
Volume2.0 mL
ImmunogenNot available
Background InformationImmunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (κ and λ; both having a molecular weight of 22.5 kDa). κ and λ consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of κ to λ is 70:30.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Franklin EC: Structure and function of immunoglobulins. Acta Endocrinol Suppl (Copenh). 1975; 194:77‑95. < PMID: 47690 > | Brinkmann V, Heusser CH: T cell‑dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec; 152(2):323‑32. < PMID: 8258141 >

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