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CyFlow™ Kappa-LC Biotin

CyFlow™ Kappa-LC Biotin
Antibody: Yes
Antigen: κ light chains
Application: Flow cytometry
Clonality: monoclonal
Clone: TB28-2
Field of Interest: Immunoglobulins
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CA388668

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ Kappa-LC Biotin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman IgG-κ myeloma protein
Background InformationImmunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (κ and λ; both having a molecular weight of 22.5 kDa). κ and λ consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of κ to λ is 60:40.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H: Monoclonal anti‑Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987 Jun 15; 138(12):4150‑8. < PMID: 3495581 > | Nakamura T, Kubagawa H, Cooper MD: Heterogeneity of immunoglobulin‑associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci USA. 1992 Sep 15; 89(18):8522‑6. < PMID: 1382292 > | Karandikar NJ, Aquino DB, McKenna RW, Kroft SH: Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome: An immunophenotypic analysis. Am J Clin Pathol. 2001 Aug; 116(2):204‑10. < PMID: 11488066 > | Böttcher S, Ritgen M, Buske S, Gesk S, Klapper W, Hoster E, Hiddemann W, Unterhalt M, Dreyling M, Siebert R, Kneba M, Pott C: EU MCL MRD Group: Minimal residual disease detection in mantle cell lymphoma: methods and significance of four‑color flow cytometry compared to consensus IGH‑polymerase chain reaction at initial staging and for follow‑up examinations. Haematologica. 2008 Apr; 93(4):551‑9. < PMID: 18379010 > | Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B: An in vitro model of differentiation of memory B cells into plasmablasts andplasma cells including detailed phenotypic and molecular characterization. Blood. 2009 Dec 10; 114(25):5173‑81. < PMID: 19846886 >

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