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CyFlow™ SIT Purified

CyFlow™ SIT Purified
Antibody: Yes
Antigen: SIT
Application: Flow cytometry, Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: SIT-01
Field of Interest: Adaptor proteins
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CZ912316

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ SIT Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenBacterially produced recombinant intracellular fragment of human SIT
Background InformationSIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B: SHP2‑interacting transmembrane adaptor protein (SIT), a novel disulfide‑linked dimer regulating human T cell activation. J Exp Med. 1999 Apr 19; 189(8):1181‑94. < PMID: 10209036 > | Hubener C, Mincheva A, Lichter P, Schraven B, Bruyns E: Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2‑interacting transmembrane adaptor protein (SIT). Immunogenetics. 2001 May-Jun; 53(4):337‑41. < PMID: 11491537 > | Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug; 4(8):603‑16. < PMID: 15286727 > | Simeoni L, Posevitz V, Kolsch U, Meinert I, Bruyns E, Pfeffer K, Reinhold D, Schraven B: The transmembrane adapter protein SIT regulates thymic development and peripheral T‑cell functions. Mol Cell Biol. 2005 Sep; 25(17):7557‑68. < PMID: 16107703 > | Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid‑cell markers. Blood. 2006 Jan 1; 107(1):213‑21. < PMID: 16160011 >

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