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CyFlow™ SIT PE

CyFlow™ SIT PE
Antibody: Yes
Antigen: SIT
Application: Flow cytometry
Clonality: monoclonal
Clone: SIT-01
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Adaptor proteins
Format/Fluorochrome: PE
Isotype: IgG1
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BJ327694

For Research Use Only

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Description
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
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CyFlow™ SIT PE
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenBacterially produced recombinant intracellular fragment of human SIT
Background InformationSIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B: SHP2‑interacting transmembrane adaptor protein (SIT), a novel disulfide‑linked dimer regulating human T cell activation. J Exp Med. 1999 Apr 19; 189(8):1181‑94. < PMID: 10209036 > | Hubener C, Mincheva A, Lichter P, Schraven B, Bruyns E: Complete sequence, genomic organization, and chromosomal localization of the human gene encoding the SHP2‑interacting transmembrane adaptor protein (SIT). Immunogenetics. 2001 May-Jun; 53(4):337‑41. < PMID: 11491537 > | Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug; 4(8):603‑16. < PMID: 15286727 > | Simeoni L, Posevitz V, Kolsch U, Meinert I, Bruyns E, Pfeffer K, Reinhold D, Schraven B: The transmembrane adapter protein SIT regulates thymic development and peripheral T‑cell functions. Mol Cell Biol. 2005 Sep; 25(17):7557‑68. < PMID: 16107703 > | Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rudiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsi V: Transmembrane adaptor molecules: a new category of lymphoid‑cell markers. Blood. 2006 Jan 1; 107(1):213‑21. < PMID: 16160011 >

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