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CyFlow™ MHCII Azide Free

CyFlow™ MHCII Azide Free
Antibody: Yes
Antigen: MHC Class II
Application: Flow cytometry, Functional assays, Immunohistochemistry, Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: M5/114
Field of Interest: Immunophenotyping, MHC
Format/Fluorochrome: Azide Free
Isotype: IgG2b
Regulatory Status: RUO
Source Species: Rat
Target Species: Mouse
Product number: CF769709

For Research Use Only

$120.00 USD*

Price excludes any applicable taxes plus shipping costs

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ MHCII Azide Free
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenActivated C57BL/6 mouse spleen cells
Background InformationMHC (major histocompatibility complex) class II (MHCII) molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding groove between MHC class II α and β chain is open at both ends and is 15-24 amino acid residues long.
Storage BufferThe reagent is provided in azide-free phosphate buffered saline (PBS) solution, pH ≈7.4; 0.2 µm filter sterilized.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Bhattacharya A, Dorf ME, Springer TA: A shared alloantigenic determinant on Ia antigens encoded by the I‑A and I‑E subregions: evidence for I region gene duplication. J Immunol. 1981 Dec; 127(6):2488‑95. < PMID: 6170707 > | Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, Benoist C, Mathis D: Mice lacking the MHC class II‑associated invariant chain. Cell. 1993 Feb 26; 72(4):635‑48. < PMID: 7679955 > | De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC: Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J Cell Sci. 1995 Oct; 108 (10):3219‑31. < PMID: 7593283 > | Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, Förster I, Reith W: Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5‑deficient mice. Immunity. 1998 Feb; 8(2):143‑55. < PMID: 9491996 > | Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, Rudensky AY, Ossendorp F, Melief CJ, Stoorvogel W, Geuze HJ: Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J Cell Biol. 2001 Oct 1; 155(1):53‑63. < PMID: 11581285 > | Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY: Cathepsin S controls MHC class II‑mediated antigen presentation by epithelial cells in vivo. J Immunol. 2005 Feb 1; 174(3):1205‑12. < PMID: 15661874 > | Zang W, Kalache S, Lin M, Schroppel B, Murphy B: MHC Class II‑mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J Am Soc Nephrol. 2005 Dec; 16(12):3661‑8. < PMID: 16221866 > | Kuwano Y, Prazma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, Okochi H, Tamaki K, Fujimoto M, Tedder TF: CD83 influences cell‑surface MHC class II expression on B cells and other antigen‑presenting cells. Int Immunol. 2007 Aug; 19(8):977‑92. < PMID: 17804692 >

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