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CyFlow™ MHCII FITC

CyFlow™ MHCII FITC
Antibody: Yes
Antigen: MHC Class II
Application: Flow cytometry
Clonality: monoclonal
Clone: M5/114
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Immunophenotyping, MHC
Format/Fluorochrome: FITC
Isotype: IgG2b
Laser: Blue
Regulatory Status: RUO
Source Species: Rat
Target Species: Mouse
Product number: BJ649436

For Research Use Only

$235.00 USD*

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Description
Concentration Unit mg/mL Concentration 0,5 Quantity 0.1 mg Volume 0.2 mL... more
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CyFlow™ MHCII FITC
Concentration Unitmg/mL
Concentration0,5
Quantity0.1 mg
Volume0.2 mL
ImmunogenActivated C57BL/6 mouse spleen cells
Background InformationMHC (major histocompatibility complex) class II (MHCII) molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding groove between MHC class II α and β chain is open at both ends and is 15-24 amino acid residues long.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 4·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Bhattacharya A, Dorf ME, Springer TA: A shared alloantigenic determinant on Ia antigens encoded by the I‑A and I‑E subregions: evidence for I region gene duplication. J Immunol. 1981 Dec; 127(6):2488‑95. < PMID: 6170707 > | Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, Benoist C, Mathis D: Mice lacking the MHC class II‑associated invariant chain. Cell. 1993 Feb 26; 72(4):635‑48. < PMID: 7679955 > | De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC: Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J Cell Sci. 1995 Oct; 108 (10):3219‑31. < PMID: 7593283 > | Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, Förster I, Reith W: Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5‑deficient mice. Immunity. 1998 Feb; 8(2):143‑55. < PMID: 9491996 > | Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, Rudensky AY, Ossendorp F, Melief CJ, Stoorvogel W, Geuze HJ: Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J Cell Biol. 2001 Oct 1; 155(1):53‑63. < PMID: 11581285 > | Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY: Cathepsin S controls MHC class II‑mediated antigen presentation by epithelial cells in vivo. J Immunol. 2005 Feb 1; 174(3):1205‑12. < PMID: 15661874 > | Zang W, Kalache S, Lin M, Schroppel B, Murphy B: MHC Class II‑mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J Am Soc Nephrol. 2005 Dec; 16(12):3661‑8. < PMID: 16221866 > | Kuwano Y, Prazma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, Okochi H, Tamaki K, Fujimoto M, Tedder TF: CD83 influences cell‑surface MHC class II expression on B cells and other antigen‑presenting cells. Int Immunol. 2007 Aug; 19(8):977‑92. < PMID: 17804692 >

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