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CyFlow™ HLA-Class I Biotin

CyFlow™ HLA-Class I Biotin
Antibody: Yes
Antigen: HLA-Class I
Application: Flow cytometry
Clonality: monoclonal
Clone: W6/32
Field of Interest: MHC
Format/Fluorochrome: Biotin
Isotype: IgG2a
Regulatory Status: RUO
Source Species: Mouse
Target Species: Cat, Cow, Human, Non-Human Primates
Product number: AK363888

For Research Use Only

$160.00 USD*

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ HLA-Class I Biotin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenMembrane of human tonsil cells
Background InformationHLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant β2-microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 3.3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A: Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens ‑ new tools for genetic analysis. Cell. 1978; 14:9‑20. < PMID: 667938 > | Brodsky FM, Parham P: Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics. 1982; 15:151‑166. < PMID: 6174442 > | Neefjes JJ, Hensen EJ, de Kroon TI, Ploegh HL: A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics. 1986; 23:341‑347. < PMID: 3458670 > | Stern PL, Beresford N, Friedman CI, Stevens VC, Risk JM, Johnson PM: Class I‑like MHC molecules expressed by baboon placental synctiotrophoblast. J Immunol. 1987; 138(4):1088‑1091. < PMID: 3543121 > | Kievits F, Ivanyi P: Monomorphic anti‑HLA monoclonal antibody (W6/32) recognizes polymorphic H‑2 heavy‑chain determinants exposed by association with bovine or human but not murine beta 2‑microglobulin.. Hum Immunol. 1987 Oct; 20(2):115‑26. < PMID: 3316135 > | Jacobsen CN, Aasted B, Broe MK, Petersen JL: Reactivities of 20 anti‑human monclonal antibodies with leucocytes from ten different animal species. Vet Immunopathol. 1993; 39:461‑466. < PMID: 8116221 > | Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta‑microglobulin. Tissue Antigens . 1998 May; 51(5):567‑70. < PMID: 9672158 > | Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P: Residue 3 of beta2‑microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999 Apr; 49(4):312‑20. < PMID: 10079295 > | Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan‑HLA class I‑reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA‑B27/beta2‑microglobulin complex. Immunogenetics. 2001 Aug; 53(6):440‑6. < PMID: 11685454 > | Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA‑G in human cornea, an immune‑privileged tissue. Hum Immunol. 2003 Nov; 64(11):1039‑44. < PMID: 14602233 >