CyFlow™ HLA-Class I FITC
| Antibody: | Yes |
| Antigen: | HLA-Class I |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | W6/32 |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Field of Interest: | MHC |
| Format/Fluorochrome: | FITC |
| Isotype: | IgG2a |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Cat, Cow, Human, Non-Human Primates |
| Product number: | BX154902 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Membrane of human tonsil cells |
| Background Information | HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant β2-microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules. |
| Usage | The reagent is designed for Flow Cytometry analysis. Suggested working usage is 3.3·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A: Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens ‑ new tools for genetic analysis. Cell. 1978; 14:9‑20. < PMID: 667938 > | Brodsky FM, Parham P: Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics. 1982; 15:151‑166. < PMID: 6174442 > | Neefjes JJ, Hensen EJ, de Kroon TI, Ploegh HL: A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics. 1986; 23:341‑347. < PMID: 3458670 > | Stern PL, Beresford N, Friedman CI, Stevens VC, Risk JM, Johnson PM: Class I‑like MHC molecules expressed by baboon placental synctiotrophoblast. J Immunol. 1987; 138(4):1088‑1091. < PMID: 3543121 > | Kievits F, Ivanyi P: Monomorphic anti‑HLA monoclonal antibody (W6/32) recognizes polymorphic H‑2 heavy‑chain determinants exposed by association with bovine or human but not murine beta 2‑microglobulin.. Hum Immunol. 1987 Oct; 20(2):115‑26. < PMID: 3316135 > | Jacobsen CN, Aasted B, Broe MK, Petersen JL: Reactivities of 20 anti‑human monclonal antibodies with leucocytes from ten different animal species. Vet Immunopathol. 1993; 39:461‑466. < PMID: 8116221 > | Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta‑microglobulin. Tissue Antigens . 1998 May; 51(5):567‑70. < PMID: 9672158 > | Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P: Residue 3 of beta2‑microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999 Apr; 49(4):312‑20. < PMID: 10079295 > | Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan‑HLA class I‑reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA‑B27/beta2‑microglobulin complex. Immunogenetics. 2001 Aug; 53(6):440‑6. < PMID: 11685454 > | Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA‑G in human cornea, an immune‑privileged tissue. Hum Immunol. 2003 Nov; 64(11):1039‑44. < PMID: 14602233 >