CyFlow™ HLA-G Biotin
| Antibody: | Yes |
| Antigen: | HLA-G |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-G/9 |
| Field of Interest: | MHC |
| Format/Fluorochrome: | Biotin |
| Isotype: | IgG1 |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AU490436 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 1 |
| Quantity | 0.1 mg |
| Volume | 0.1 mL |
| Immunogen | Recombinant human HLA-G refolded with β2-microglobulin and peptide |
| Usage | The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 1·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Fournel S, Huc X, Aguerre-Girr M, Solier C, Legros M, Praud-Brethenou C, Moussa M, Chaouat G, Berrebi A, Bensussan A, Lenfant F, Le Bouteiller P: Comparative reactivity of different HLA‑G monoclonal antibodies to soluble HLA‑G molecules. Tissue Antigens. 2000 Jun; 55(6):510‑8. < PMID: 10902607 > | Fuzzi B, Rizzo R, Criscuoli L, Noci I, Melchiorri L, Scarselli B, Bencini E, Menicucci A, Baricordi OR: HLA‑G expression in early embryos is a fundamental prerequisite for the obtainment of pregnancy. Eur J Immunol. 2002 Feb; 32(2):311‑5. < PMID: 11807769 > | Pangault C, Le Friec G, Caulet-Maugendre S, Lena H, Amiot L, Guilloux V, Onno M, Fauchet R: Lung macrophages and dendritic cells express HLA‑G molecules in pulmonarydiseases. Hum Immunol. 2002 Feb; 63(2):83‑90. < PMID: 11821155 > | Lozano JM, Gonzalez R, Kindelan JM, Rouas-Freiss N, Caballos R, Dausset J, Carosella ED, Pena J: Monocytes and T lymphocytes in HIV‑1‑positive patients express HLA‑G molecule. AIDS. 2002 Feb 15; 16(3):347‑51. < PMID: 11834945 > | Abstracts from the 3rd International Conference on HLA‑G; Paris, July 2003. Tissue Antigens. 2003; 62(4):340‑57. < Wiley j.1399-0039 > | Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar; 64(3):315‑26. < PMID: 12590976 > | Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor‑1 distinguishes between conformed and beta 2‑microglobulin‑free HLA‑G molecules. J Immunol. 2005 Oct 15; 175(8):4866‑74. < PMID: 16210588 > | Lopez AS, Alegre E, LeMaoult J, Carosella E, Gonzalez A: Regulatory role of tryptophan degradation pathway in HLA‑G expression by human monocyte‑derived dendritic cells. Mol Immunol. 2006 Jul; 43(14):2151‑60. < PMID: 16490253 > | Zhao L, Teklemariam T, Hantash BM: Reassessment of HLA‑G isoform specificity of MEM‑G/9 and 4H84 monoclonal antibodies. Tissue Antigens. 2012 Sep; 80(3):231‑8. < PMID: 22738368 >