CyFlow™ HLA-G PE
| Antibody: | Yes |
| Antigen: | HLA-G |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | 87G |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | MHC |
| Format/Fluorochrome: | PE |
| Isotype: | IgG2a |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BF694972 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 0,1 |
| Quantity | 0.1 mg |
| Volume | 1.0 mL |
| Immunogen | HLA-B27 transgenic mice were immunized with H-2 identical mouse cells transfected with and expressing genes encoding HLA-G and human β2-microglobulin |
| Usage | The reagent is designed for Flow Cytometry analysis. Suggested working usage is 10·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Lee N, Malacko AR, Ishitani A, Chen MC, Bajorath J, Marquardt H, Geraghty DE: The membrane‑bound and soluble forms of HLA‑G bind identical sets of endogenous peptides but differ with respect to TAP association. Immunity. 1995; 3(5):591‑600. < PMID: 7584149 > | Riteau B, Menier C, Khalil-Daher I, Martinozzi S, Pla M, Dausset J, Carosella ED, Rouas-Freiss N: HLA‑G1 co‑expression boosts the HLA class I‑mediated NK lysis inhibition. Int Immunol. 2001 Feb; 13(2):193‑201. < PMID: 11157852 > | Ishitani A, Sageshima N, Lee N, Dorofeeva N, Hatake K, Marquardt H, Geraghty DE: Protein Expression and Peptide Binding Suggest Unique and Interacting Functional Roles for HLA‑E, F and G in Maternal‑Placental Immune Recognitions. J Immunol. 2003; 171:1376. < PMID: 12874228 > | Polakova K, Bandzuchova E, Hofmeister V, Weiss EH, Hutter H, Russ G: Binding analysis of HLA‑G specific antibodies to hematopoietic cells isolated from leukemia patients. Neoplasma. 2003; 50(5):331‑8. < PMID: 14628085 > | Wiendl H: The non‑classical MHC molecule HLA‑G protects human muscle cells from immune‑mediated lysis: implications for myoblast transplantation and gene therapy. Brain. 2003; 126(1):176‑85. < PMID: 12477705 > | Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar; 64(3):315‑26. < PMID: 12590976 > | Sageshima N, Ishitani A, Omura M, Akasaki M, Umekage H, Katabuchi H, Okamura H, Hatake K: Necrotic feature of the trophoblasts lacking HLA‑G expression in normal andpre‑eclamptic placentas. Am J Reprod Immunol. 2003 Mar; 49(3):174‑82. < PMID: 12797524 > | Polakova K, Krcova M, Kuba D, Russ G: Analysis of HLA‑G expression in malignant hematopoetic cells from leukemia patients. Leuk Res. 2003 Jul; 27(7):643‑8. < PMID: 12681364 > | Hackmon R, Hallak M, Krup M, Weitzman D, Sheiner E, Kaplan B, Weinstein Y: HLA‑G antigen and parturition: maternal serum, fetal serum and amniotic fluid levels during pregnancy. Fetal Diagn Ther. 2004 Sep-Oct; 19(5):404‑9. < PMID: 15305096 > | Rouas-Freiss N, Moreau P, Ferrone S, Carosella ED: HLA‑G proteins in cancer: do they provide tumor cells with an escape mechanism?. Cancer Res. 2005 Nov 15; 65(22):10139‑44. < PMID: 16287995 > | Shobu T, Sageshima N, Tokui H, Omura M, Saito K, Nagatsuka Y, Nakanishi M, Hayashi Y, Hatake K, Ishitani A: The surface expression of HLA‑F on decidual trophoblasts increases from mid to term gestation. J Reprod Immunol. 2006 Dec; 72(1‑2):18‑32. < PMID: 16806485 > | LeMaoult J, Caumartin J, Daouya M, Favier B, Le Rond S, Gonzalez A, Carosella ED: Immune regulation by pretenders: cell‑to‑cell transfers of HLA‑G make effector T cells act as regulatory cells. Blood. 2007 Mar 1; 109(5):2040‑8. < PMID: 17077329 >