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CyFlow™ HLA-G Biotin

CyFlow™ HLA-G Biotin
Antibody: Yes
Antigen: HLA-G
Application: Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Western blot
Clonality: monoclonal
Clone: 5A6G7
Field of Interest: MHC
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BV752615

For Research Use Only

$160.00 USD*

Price excludes any applicable taxes plus shipping costs

Description Documentation TDS & SDS
Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ HLA-G Biotin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenC-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry and for Western blotting, ELISA and Immunocytochemistry. Suggested working usage is 1·µg/ml (for Western blotting). Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA‑G or HLA‑E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar; 64(3):315‑26. < PMID: 12590976 > | Le Rond S, Le Maoult J, Creput C, Menier C, Deschamps M, Le Friec G, Amiot L, Durrbach A, Dausset J, Carosella ED, Rouas-Freiss N: Alloreactive CD4+ and CD8+ T cells express the immunotolerant HLA‑G molecule in mixed lymphocyte reactions: in vivo implications in transplanted patients. Eur J Immunol. 2004 Mar; 34(3):649‑60. < PMID: 14991594 > | Rebmann V, Lemaoult J, Rouas-Freiss N, Carosella ED, Grosse-Wilde H: Report of the Wet Workshop for Quantification of Soluble HLA‑G in Essen, 2004. Hum Immunol. 2005 Aug; 66(8):853‑63. < PMID: 16216668 > | Le Rond S, Gonzalez A, Gonzalez AS, Carosella ED, Rouas-Freiss N: Indoleamine 2,3 dioxygenase and human leucocyte antigen‑G inhibit the T‑cell alloproliferative response through two independent pathways. Immunology. 2005 Nov; 116(3):297‑307. < PMID: 16236119 > | Lafon M, Prehaud C, Megret F, Lafage M, Mouillot G, Roa M, Moreau P, Rouas-Freiss N, Carosella ED: Modulation of HLA‑G Expression in Human Neural Cells after Neurotropic Viral Infections. J Virol. 2005 Dec; 79(24):15226‑15237. < PMID: 16306594 > | Poláková K, Železníková T, Russ G: HLA‑G5 in the blood of leukemia patients and healthy individuals. Leuk Res. 2013 Feb; 37(2):139‑45. < PMID: 23146557 >

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