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CyFlow™ HLA-E Biotin

CyFlow™ HLA-E Biotin
Antibody: Yes
Antigen: HLA-E
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-E/07
Field of Interest: MHC
Format/Fluorochrome: Biotin
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AU515518

For Research Use Only

$160.00 USD*

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
CyFlow™ HLA-E Biotin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenBacterially expressed recombinant HLA-E refolded with β2-microglobulin and peptide
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 2·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Sibilio L, et al.: Abstract 45 from the 3rd International Conference on HLA‑G; Paris, July 2003. Tissue Antigens. 2003; 62:339. < No PMID > | Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pistillo MP: HLA‑E surface expression is independent of the availability of HLA class I signal sequence‑derived peptides in human tumor cell lines. Hum Immunol. 2005 Jan; 66(1):42705. < PMID: 15620456 > | Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep; 13(9):822‑30. < PMID: 21969815 > | Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le VT, Galun E, Horejsi V, Isakov O, Shomron N, Wolf DG, Hengel H, Mandelboim O: MicroRNA editing facilitates immune elimination of HCMV infected cells. PLoS Pathog. 2014 Feb 27; 10(2):e1003963. < PMID: 24586166 >