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CyFlow™ HLA-DR1 (empty) PE

CyFlow™ HLA-DR1 (empty) PE
Antibody: Yes
Antigen: HLA-DR1 (empty)
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-267
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: MHC
Format/Fluorochrome: PE
Isotype: IgG2b
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CM093224

For Research Use Only

$255.00 USD*

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Description
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
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CyFlow™ HLA-DR1 (empty) PE
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenPurified, insoluble DR1 β chain (DRB1*0101) expressed in E. coli inclusion bodies
Background InformationHLA-DR1 belongs to the HLA class II β chain paralogues. The MHC Class II molecule is a heterodimer consisting of an α (DRA) and a β chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The β chain is approximately 26-28 kDa. Within the DR molecule the β chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Carven GJ, Chitta S, Hilgert I, Rushe MM, Baggio RF, Palmer M, Arenas JE, Strominger JL, Horejsi V, Santambrogio L, Stern LJ: Monoclonal antibodies specific for the empty conformation of HLA‑DR1 reveal aspects of the conformational change associated with peptide binding. J Biol Chem. 2004 Apr 16; 279(16):16561‑70. < PMID: 14757758 > | Potolicchio I, Chitta S, Xu X, Fonseca D, Crisi G, Horejsi V, Strominger JL, Stern LJ, Raposo G, Santambrogio L: Conformational variation of surface class II MHC proteins during myeloid dendritic cell differentiation accompanies structural changes in lysosomal MIIC. J Immunol. 2005 Oct 15; 175(8):4935‑47. < PMID: 16210595 >

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