CyFlow™ HLA-B7 Purified
Antibody: | Yes |
Antigen: | HLA-B7 |
Application: | Flow cytometry |
Clonality: | monoclonal |
Clone: | BB7.1 |
Field of Interest: | MHC |
Format/Fluorochrome: | Purified |
Isotype: | IgG1 |
Regulatory Status: | RUO |
Source Species: | Mouse |
Target Species: | Human, Non-Human Primates |
Product number: | AF692459 |
For Research Use Only
Concentration Unit | mg/mL |
Concentration | 1 |
Quantity | 0.1 mg |
Volume | 0.1 mL |
Immunogen | Papain solubilised HLA-A2, B7 |
Background Information | HLA-B7 allele of human HLA class I major histocompatibility (MHC) antigen indicates higher risk of breast cancer and cervical cancer. Expression of HLA-B7 together with HLA-B27 is associated with increased susceptibility to spondyloarthropaties. Flow cytometry detection of these two alleles is being used to screen for patients, who suffer from inflammatory disorders affecting the sacroiliac and intervertebral joints, such as ankylosing spondylosis (AS). The HLA-B7 antigen (11 alleles) is expressed in 22% of healthy Caucasian individuals. |
Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
Stability | Do not use after expiration date stamped on vial label. |
| Trapani JA, Vaughan HA, Tait BD, McKenzie IF: Immunoradiometric assay for the rapid detection of HLA‑B27. Immunol Cell Biol. 1988 Jan; 66 ( 3):215‑9. < PMID: 3155158 > | Storkus WJ, Alexander J, Payne JA, Dawson JR, Cresswell P: Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc Natl Acad Sci USA. 1989 Apr; 86(7):2361‑4. < PMID: 2784569 > | Nabel GJ, Nabel EG, Yang ZY, Fox BA, Plautz GE, Gao X, Huang L, Shu S, Gordon D, Chang AE: Direct gene transfer with DNA‑liposome complexes in melanoma: expression, biologic activity, and lack of toxicity in humans. Proc Natl Acad Sci USA. 1993 Dec 1; 90(23):11307‑11. < PMID: 8248244 > | Rini BI, Selk LM, Vogelzang NJ: Phase I study of direct intralesional gene transfer of HLA‑B7 into metastatic renal carcinoma lesions. Clin Cancer Res. 1999 Oct; 5(10):2766‑72. < PMID: 10537340 > | Worley BS, van den Broeke LT, Goletz TJ, Pendleton CD, Daschbach EM, Thomas EK, Marincola FM, Helman LJ, Berzofsky JA: Antigenicity of fusion proteins from sarcoma‑associated chromosomal translocations. Cancer Res. 2001 Sep 15; 61(18):6868‑75. < PMID: 11559563 > | Wang SS, Wheeler CM, Hildesheim A, Schiffman M, Herrero R, Bratti MC, Sherman ME, Alfaro M, Hutchinson ML, Morales J, Lorincz A, Burk RD, Carrington M, Erlich HA, Apple RJ: Human leukocyte antigen class I and II alleles and risk of cervical neoplasia: results from a population‑based study in Costa Rica. J Infect Dis. 2001 Nov 15; 184(10):1310‑4. < PMID: 11679920 > | de la Salle H, Saulquin X, Mansour I, Klayme S, Fricker D, Zimmer J, Cazenave JP, Hanau D, Bonneville M, Houssaint E, Lefranc G, Naman R: Asymptomatic deficiency in the peptide transporter associated to antigen processing (TAP). Clin Exp Immunol. 2002 Jun; 128(3):525‑31. < PMID: 12067308 > | Grunewald J, Eklund A, Olerup O: Human leukocyte antigen class I alleles and the disease course in sarcoidosis patients. Am J Respir Crit Care Med. 2004 Mar 15; 169(6):696‑702. < PMID: 14656748 > | Hofmann S, Glückmann M, Kausche S, Schmidt A, Corvey C, Lichtenfels R, Huber C, Albrecht C, Karas M, Herr W: Rapid and sensitive identification of major histocompatibility complex class I‑associated tumor peptides by Nano‑LC MALDI MS/MS. Mol Cell Proteomics. 2005 Dec; 4(12):1888‑97. < PMID: 16112985 > | Cortez-Gonzalez X, Sidney J, Adotevi O, Sette A, Millard F, Lemonnier F, Langlade-Demoyen P, Zanetti M: Immunogenic HLA‑B7‑restricted peptides of hTRT. Int Immunol. 2006 Dec; 18(12):1707‑18. < PMID: 17077179 > | Bhattacharya P, Sengupta S: Predisposition to HPV16/18‑related cervical cancer because of proline homozygosity at codon 72 of p53 among Indian women is influenced by HLA‑B*07 and homozygosity of HLA‑DQB1*03. Tissue Antigens. 2007 Oct; 70(4):283‑93. < PMID: 17767549 > | Harjacek M, Margetić T, Kerhin-Brkljacić V, Martinez N, Grubić Z: HLA‑B*27/HLA‑B*07 in combination with D6S273‑134 allele is associated with increased susceptibility to juvenile spondyloarthropathies. Clin Exp Rheumatol. 2008 May-Jun; 26(3):498‑504. < PMID: 18578977 > | Lo Monaco E, Sibilio L, Melucci E, Tremante E, Suchànek M, Horejsi V, Martayan A, Giacomini P: HLA‑E: strong association with beta2‑microglobulin and surface expression in the absence of HLA class I signal sequence‑derived peptides. Immunol. 2008 Oct 15; 181(8):5442‑50. < PMID: 18832701 >