CyFlow™ Helios PE
| Alternative Name: | IKZF2 |
| Antibody: | Yes |
| Antigen: | Helios |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | 22F6 |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Transcription |
| Format/Fluorochrome: | PE |
| Isotype: | IgG |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Hamster |
| Target Species: | Human, Mouse |
| Product number: | CV254145 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Peptide coresponding to the amino acids 51-107 of Helios |
| Background Information | Helios (IKZF2; Ikaros family zinc finger protein 2) is a hematopoietic-specific transcription factor involved in the regulation of lymphocyte development, together with other members of this family, such as Aiolos and Ikaros. Helios forms homo- and heterodimers with these proteins and is thought to function predominantly in early hematopoietic development. Expression of Helios, Aiolos and Ikaros is restricted to cells of the hematopoietic system, whereas other family members, Eos and Pegassus, are more widely expressed. Helios is expressed at early stages of thymocyte development. In mature T cells, Helios has been strongly associated with Treg cells. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Thornton AM, Korty PE, Tran DQ, Wohlfert EA, Murray PE, Belkaid Y, Shevach EM: Expression of Helios, an Ikaros transcription factor family member, differentiates thymic‑derived from peripherally induced Foxp3+ T regulatory cells. J Immunol. 2010 Apr 1; 184(7):3433‑41. < PMID: 20181882 > | Pinheiro D, Singh Y, Grant CR, Appleton RC, Sacchini F, Walker KR, Chadbourne AH, Palmer CA, Armitage-Chan E, Thompson I, Williamson L, Cunningham F, Garden OA: Phenotypic and functional characterization of a CD4(+) CD25(high) FOXP3(high) regulatory T‑cell population in the dog. Immunology. 2011 Jan; 132(1):111‑22. < PMID: 20880379 > | Serre K, Bénézech C, Desanti G, Bobat S, Toellner KM, Bird R, Chan S, Kastner P, Cunningham AF, Maclennan IC, Mohr E: Helios is associated with CD4 T cells differentiating to T helper 2 and follicular helper T cells in vivo independently of Foxp3 expression. PLoS One. 2011; 6(6):e20731. < PMID: 25877073 > | Daley SR, Hu DY, Goodnow CC: Helios marks strongly autoreactive CD4+ T cells in two major waves of thymic deletion distinguished by induction of PD‑1 or NF‑κB. J Exp Med. 2013 Feb 11; 210(2):269‑85. < PMID: 23337809 > | McIver Z, Melenhorst JJ, Wu C, Grim A, Ito S, Cho I, Hensel N, Battiwalla M, Barrett AJ: Donor lymphocyte count and thymic activity predict lymphocyte recovery and outcomes after matched‑sibling hematopoietic stem cell transplant. Haematologica. 2013 Mar; 98(3):346‑52. < PMID: 23065508 > | Atarashi K, Tanoue T, Oshima K, Suda W, Nagano Y, Nishikawa H, Fukuda S, Saito T, Narushima S, Hase K, Kim S, Fritz JV, Wilmes P, Ueha S, Matsushima K, Ohno H, Olle B, Sakaguchi S, Taniguchi T, Morita H, Hattori M, Honda K: Treg induction by a rationally selected mixture of Clostridiastrains from the human microbiota. Nature. 2013 Aug 8; 500(7461):232‑6. < PMID: 23842501 > | Blankenhaus B, Reitz M, Brenz Y, Eschbach ML, Hartmann W, Haben I, Sparwasser T, Huehn J, Kühl A, Feyerabend TB, Rodewald HR, Breloer M: Foxp3+ regulatory T cells delay expulsion of intestinal nematodes by suppression of IL‑9‑driven mast cell activation in BALB/c but not in C57BL/6 mice. PLoS Pathog. 2014 Feb 6; 10(2):e1003913. < PMID: 24516385 >