CyFlow™ GCPII PE
| Alternative Name: | PSMA |
| Antibody: | Yes |
| Antigen: | GCPII |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | GCP-05 |
| Emission Maximum: | 576 nm |
| Excitation Maximum: | 496 nm, 565 nm |
| Field of Interest: | Neurobiology |
| Format/Fluorochrome: | PE |
| Isotype: | IgG1 |
| Laser: | Blue , Green, Yellow |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BR339703 |
For Research Use Only
| Concentration Unit | mg/mL |
| Concentration | 0,1 |
| Quantity | 0.1 mg |
| Volume | 1.0 mL |
| Immunogen | Amino acids 44-750 of human GCPII |
| Background Information | Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-α-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1) and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-γ-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumors and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation. |
| Usage | The reagent is designed for Flow Cytometry analysis. Suggested working usage is 0.5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Barinka C, Rinnova M, Sacha P, Rojas C, Majer P, Slusher BS, Konvalinka J: Substrate specificity, inhibition and enzymological analysis of recombinant human glutamate carboxypeptidase II. J Neurochem. 2002; 80:477‑487. < PMID: 11905994 > | Ghadge GD, Slusher BS, Bodner A, Canto MD, Wozniak K, Thomas AG, Rojas C, Tsukamoto T, Majer P, Miller RJ, Monti AL, Roos RP: Glutamate carboxypeptidase II inhibition protects motor neurons from death in familial amyotrophic lateral sclerosis models. Proc Natl Acad Sci USA. 2003 Aug 5; 100(16):9554‑9. < PMID: 12876198 > | Barinka C, Mlcochova P, Sacha P, Hilgert I, Majer P, Slusher BS, Horejsi V, Konvalinka J: Amino acids at the N‑ and C‑termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding. Eur J Biochem. 2004; 271:2782‑2790. < PMID: 15206943 > | Barinka C, Sacha P, Sklenar J, Man P, Bezouska K, Slusher BS, Konvalinka J: Identification of the N‑glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity. Protein Sci. 2004; 13:1627‑1635. < PMID: 15152093 > | Davis MI, Bennett MJ, Thomas LM, Bjorkman PJ: Crystal structure of prostate‑specific membrane antigen, a tumor marker and peptidase. Proc Natl Acad Sci USA. 2005; 102:5981‑5986. < PMID: 15837926 > | Mesters JR, Barinka C, Li W, Tsukamoto T, Majer P, Slusher BS, Konvalinka J, Hilgenfeld R: Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer. EMBO J. 2006; 1375‑1384. < PMID: 16467855 > | Sacha P, Zamecnik J, Barinka C, Hlouchova K, Vicha A, Mlcochova P, Hilgert I, Eckschlager T, Konvalinka J: Expression of glutamate carboxypeptidase II in human brain. Neuroscience. 2007; 144:1361‑1372. < PMID: 17150306 > | Tykvart J, Navrátil V, Sedlák F, Corey E, Colombatti M, Fracasso G, Koukolík F, Bařinka C, Sácha P, Konvalinka J: Comparative analysis of monoclonal antibodies against prostate‑specific membrane antigen (PSMA). Prostate. 2014 Sep 27; < PMID: 26629066 >