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CyFlow™ Cyclin D1 Purified

CyFlow™ Cyclin D1 Purified
Antibody: Yes
Antigen: Cyclin D1
Application: Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western blot
Clonality: monoclonal
Clone: CD1.1
Field of Interest: Signaling, Transcription
Format/Fluorochrome: Purified
Isotype: IgG1
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Rat
Product number: CV029559

For Research Use Only

$120.00 USD*

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ Cyclin D1 Purified
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenPurified cyclin D1 protein
Background InformationCyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Diehl JA, Zindy F, Sherr CJ: Inhibition of cyclin D1 phosphorylation on threonine‑286 prevents its rapid degradation via the ubiquitin‑proteasome pathway. Genes Dev. 1997 Apr 15; 11(8):957‑72. < PMID: 9136925 > | Diehl JA, Cheng M, Roussel MF, Sherr CJ: Glycogen synthase kinase‑3beta regulates cyclin D1 proteolysis and subcellular localization. Genes Dev. 1998 Nov 15; 12(22):3499‑511. < PMID: 9832503 > | Yang W, Zhang Y, Li Y, Wu Z, Zhu D: Myostatin induces cyclin D1 degradation to cause cell cycle arrest through a phosphatidylinositol 3‑kinase/AKT/GSK‑3 beta pathway and is antagonized by insulin‑like growth factor 1. J Biol Chem. 2007 Feb 9; 282(6):3799‑808. < PMID: 17130121 > | Mukherji A, Janbandhu VC, Kumar V: GSK‑3beta‑dependent destabilization of cyclin D1 mediates replicational stress‑induced arrest of cell cycle. FEBS Lett. 2008 Mar 6; < PMID: 23416295 >

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