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CyFlow™ CK-19 Alexa Fluor™ 488

CyFlow™ CK-19 Alexa Fluor™ 488
Antibody: Yes
Antigen: Cytokeratin 19
Application: Flow cytometry
Clonality: monoclonal
Clone: A53-B/A2
Emission Maximum: 519 nm
Excitation Maximum: 495 nm
Field of Interest: Cytoskeleton
Format/Fluorochrome: Alexa Fluor™ 488
Isotype: IgG2a
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BF824641

For Research Use Only

$285.00 USD*

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Description Documentation TDS & SDS
Description
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
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CyFlow™ CK-19 Alexa Fluor™ 488
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenMCF-7 human breast adenocarcinoma cell line
Background InformationCytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 10·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H: Monoclonal anti‑cytokeratin antibody from a hybridoma clone generated by electrofusion. Eur J Cancer Clin Oncol. 1985 Jun; 21(6):733‑40. < PMID: 2410280 > | Bártek J, Bártková J, Taylor-Papadimitriou J, Rejthar A, Kovarík J, Lukás Z, Vojtĕsek B: Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem J. 1986 Oct; 18(10):565‑75. < PMID: 2433255 > | Kasper M, Moll R, Stosiek P, Karsten U: Patterns of cytokeratin and vimentin expression in the human eye. Histochemistry. 1988; 89(4):369‑77. < PMID: 2457569 >

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