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CyFlow™ CK-18 FITC

CyFlow™ CK-18 FITC
Antibody: Yes
Antigen: Cytokeratin 18
Application: Flow cytometry
Clonality: monoclonal
Clone: DC-10
Emission Maximum: 518 nm
Excitation Maximum: 490 to 495 nm
Field of Interest: Cytoskeleton
Format/Fluorochrome: FITC
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AH304191

For Research Use Only

$235.00 USD*

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Description Documentation TDS & SDS
Description
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
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CyFlow™ CK-18 FITC
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenHuman breast cancer PMC-42 cells
Background InformationCytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
UsageThe reagent is designed for Flow Cytometry analysis. Suggested working usage is 7.5·µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988; 3:50‑5. < PMID: 2463228 > | Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct; 7(5):495‑504. < PMID: 2461901 > | Vojtĕsek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989; 35(6):373‑82. < PMID: 2483834 >

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