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CyFlow™ CK-18 Alexa Fluor™ 488

CyFlow™ CK-18 Alexa Fluor™ 488
Antibody: Yes
Antigen: Cytokeratin 18
Application: Flow cytometry
Clonality: monoclonal
Clone: C-04
Emission Maximum: 519 nm
Excitation Maximum: 495 nm
Field of Interest: Cytoskeleton
Format/Fluorochrome: Alexa Fluor™ 488
Isotype: IgG1
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Mammalian
Product number: AP635076

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Description
Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
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CyFlow™ CK-18 Alexa Fluor™ 488
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenCytoskeleton preparation of epidermal carcinoma cell line A431
Background InformationCytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988; 3:50‑5. < PMID: 2463228 > | Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct; 7(5):495‑504. < PMID: 2461901 > | Vojtĕsek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989; 35(6):373‑82. < PMID: 2483834 > | Taylor-Papadimitriou J, Stampfer M, Bartek J, Lewis A, Boshell M, Lane EB, Leigh IM: Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci. 1989 Nov; 94 ( 3):403‑13. < PMID: 2483723 > | Kovarik J, et al: J Tumor Marker Oncol. 1990; 5:219. < No PMID > | Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul; 164(3):215‑24. < PMID: 1716305 > | Cap J, Cerman J, Nemecek S, Marekova M, Hana V, Frysak Z: The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH‑secreting pituitary adenomas. J Clin Neurosci. 2003 Jul; 10(4):444‑8. < PMID: 12852883 >

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