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CyFlow™ CD326 Alexa Fluor™ 647

CyFlow™ CD326 Alexa Fluor™ 647
Alternative Name: EGP, EGP-40, EpCAM, hEGP-2, M4S1, MIC18, MK1, TACSTD1 , TROP1
Antibody: Yes
Antigen: CD326
Application: Flow cytometry
Clonality: monoclonal
Clone: VU-1D9
Emission Maximum: 665 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 647
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AD559349

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 0.4 mL Immunogen Small cell lung carcinoma cell line H69... more
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CyFlow™ CD326 Alexa Fluor™ 647
Quantity100 tests
Volume0.4 mL
ImmunogenSmall cell lung carcinoma cell line H69
Background InformationCD326 (EpCAM, ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumors and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Tsubura A, Senzaki H, Sasaki M, Hilgers J, Morii S: Immunohistochemical demonstration of breast‑derived and/or carcinoma‑associated glycoproteins in normal skin appendages and their tumors. J Cutan Pathol. 1992 Feb; 19(1):73‑9. < PMID: 1556271 > | Ogura E, Senzaki H, Yoshizawa K, Hioki K, Tsubura A: Immunohistochemical localization of epithelial glycoprotein EGP‑2and carcinoembryonic antigen in normal colonic mucosa and colorectal tumors. Anticancer Res. 1998 Sep-Oct; 18(5B):3669‑75. < PMID: 9854475 > | Li G, Passebosc-Faure K, Lambert C, Gentil-Perret A, Blanc F, Oosterwijk E, Mosnier JF, Genin C, Tostain J: Flow cytometric analysis of antigen expression in malignant and normal renal cells. Anticancer Res. 2000 Jul-Aug; 20(4):2773‑8. < PMID: 10953356 > | Winter MJ, Nagtegaal ID, van Krieken JH, Litvinov SV: The epithelial cell adhesion molecule (Ep‑CAM) as a morphoregulatory molecule is a tool in surgical pathology. Am J Pathol. 2003 Dec; 163(6):2139‑48. < PMID: 14633587 > | Brunner A, Prelog M, Verdorfer I, Tzankov A, Mikuz G, Ensinger C: EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 2008 Mar; 61(3):307‑10. < PMID: 17586680 >

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