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CyFlow™ CD314 APC

CyFlow™ CD314 APC
Alternative Name: KLAR, KLR, KLRK1, NKG2-D, NKG2D
Antibody: Yes
Antigen: CD314
Application: Flow cytometry
Clonality: monoclonal
Clone: 1D11
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BU361318

For Research Use Only

$255.00 USD*

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 1.0 mL Immunogen NKL cell line Background Information... more
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CyFlow™ CD314 APC
Quantity100 tests
Volume1.0 mL
ImmunogenNKL cell line
Background InformationCD314 (NKG2D; natural killer receptor G2D or KLRK1; killer cell lectin-like receptor subfamily K, member 1), is a homodimeric C-type lectin-like activating receptor and costimulator with type II membrane orientation (C teminus extracellular). CD314 homodimers are associated with DAP10, a membrane adaptor protein that signals similar to CD28 by recruitment of phosphatidylinositol 3-kinase. Engagement of CD314 amplifies antigen-specific T cell responses in CD314-positive T cell populations. In NK cells, CD314 is a primary activating receptor. As CD314 ligands the MHC class-I chain-related proteins A and B (MICA, MICB) and UL16-binding proteins (ULBPs) have been identified.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Bauer S, Groh V, Wu J, Steinle A, Phillips JH, Lanier LL, Spies T: Activation of NK cells and T cells by NKG2D, a receptor for stress‑inducible MICA. Science. 1999 Jul 30; 285(5428):727‑9. < PMID: 10426993 > | Wu J, Cherwinski H, Spies T, Phillips JH, Lanier LL: DAP10 and DAP12 form distinct, but functionally cooperative, receptor complexes in natural killer cells. J Exp Med. 2000 Oct 2; 192(7):1059‑68. < PMID: 11015446 > | Wu J, Groh V, Spies T: T cell antigen receptor engagement and specificity in the recognition of stress‑inducible MHC class I‑related chains by human epithelial gamma delta T cells. J Immunol. 2002 Aug 1; 169(3):1236‑40. < PMID: 12133944 > | Ebert LM, Meuter S, Moser B: Homing and function of human skin gammadelta T cells and NK cells: relevance for tumor surveillance. J Immunol. 2006 Apr 1; 176(7):4331‑6. < PMID: 16547270 > | Hasenkamp J, Borgerding A, Uhrberg M, Falk C, Chapuy B, Wulf G, Jung W, Trümper L, Glass B: Self‑tolerance of human natural killer cells lacking self‑HLA‑specific inhibitoryreceptors. Scand J Immunol. 2008 Mar; 67(3):218‑29. < PMID: 18226015 > | Sangiolo D, Martinuzzi E, Todorovic M, Vitaggio K, Vallario A, Jordaney N, Carnevale-Schianca F, Capaldi A, Geuna M, Casorzo L, Nash RA, Aglietta M, Cignetti A: Alloreactivity and anti‑tumor activity segregate within two distinct subsets of cytokine‑induced killer (CIK) cells: implications for their infusion across major HLA barriers. Int Immunol. 2008 Jul; 20(7):841‑8. < PMID: 18469328 > | Valencia J, Hernández-López C, Martínez VG, Hidalgo L, Zapata AG, Vicente A, Varas A, Sacedón R: Transient beta‑catenin stabilization modifies lineage output from human thymic CD34+CD1a‑ progenitors. J Leukoc Biol. 2010 Mar; 87(3):405‑14. < PMID: 19952356 >

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