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CyFlow™ CD235a PE-Cy5

CyFlow™ CD235a PE-Cy5
Alternative Name: Glycophorin A , GPA, MN, MNS, PAS-2
Antibody: Yes
Antigen: CD235a
Application: Flow cytometry
Clonality: monoclonal
Clone: JC159
Emission Maximum: 670 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE-Cy5
Isotype: IgG1
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Rat
Product number: AR720332

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 0.4 mL Immunogen Membrane preparation from splenic hairy... more
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CyFlow™ CD235a PE-Cy5
Quantity100 tests
Volume0.4 mL
ImmunogenMembrane preparation from splenic hairy cell leukemia
Background InformationCD235a (GPA; Glycophorin A) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Maijenburg MW, Kleijer M, Vermeul K, Mul EP, van Alphen FP, van der Schoot CE, Voermans C: The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging. Haematologica. 2012 Feb; 97(2):179‑83. < PMID: 21993672 > | Alijotas-Reig J, Palacio-Garcia C, Llurba E, Vilardell-Tarres M: Cell‑derived microparticles and vascular pregnancy complications: a systematic and comprehensive review. Fertil Steril. 2013 Feb; 99(2):441‑9. < PMID: 23122952 > | Yamauchi T, Takenaka K, Urata S, Shima T, Kikushige Y, Tokuyama T, Iwamoto C, Nishihara M, Iwasaki H, Miyamoto T, Honma N, Nakao M, Matozaki T, Akashi K: Polymorphic Sirpa is the genetic determinant for NOD‑based mouse lines to achieve efficient human cell engraftment. Blood. 2013 Feb 21; 121(8):1316‑25. < PMID: 23293079 > | Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR: Platelets and erythrocyte‑bound platelets bind infectious HIV‑1 in plasma of chronically infected patients. PLoS One. 2013 Nov 25; 8(11):e81002. < PMID: 24282562 >

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