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CyFlow™ CD222 Alexa Fluor™ 647

CyFlow™ CD222 Alexa Fluor™ 647
Alternative Name: CIMPR, IGF2R , M6P-R, MPR1
Antibody: Yes
Antigen: CD222
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-238
Emission Maximum: 665 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping, Signaling
Format/Fluorochrome: Alexa Fluor™ 647
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: AQ486061

For Research Use Only

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 0.4 mL Immunogen Recombinant Vaccinia virus encoding CD222... more
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CyFlow™ CD222 Alexa Fluor™ 647
Quantity100 tests
Volume0.4 mL
ImmunogenRecombinant Vaccinia virus encoding CD222
Background InformationCD222 (CIMPR; cation-independent mannose 6-phosphate receptor or IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-β, last but not least CD222 serves as a receptor for heparanase and even for Listeria.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Mason D, Simmons D, Buckley C, Schwartz-Albiez R, Hadam M, Saalmuller A, Clark E, Malavasi F, Morrissey JA (Eds): Leucocyte Typing VII. Oxford University Press. 2002; 1‑945. < NLM ID: 101177131 > | Leksa V, Godar S, Cebecauer M, Hilgert I, Breuss J, Weidle UH, Horejsi V, Binder BR, Stockinger H: The N terminus of mannose 6‑phosphate/insulin‑like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem. 2002 Oct 25; 277(43):40575‑82. < PMID: 12189157 > | Gasanov U, Koina C, Beagley KW, Aitken RJ, Hansbro PM: Identification of the insulin‑like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes. Infect Immun. 2006 Jan; 74(1):566‑77. < PMID: 16369013 > | Wood RJ, Hulett MD: Cell surface‑expressed cation‑independent mannose 6‑phosphate receptor (CD222 binds enzymatically active heparanase independently of mannose 6‑phosphate to promote extracellular matrix degradation. J Biol Chem. 2008 Feb 15; 283(7):4165‑76. < PMID: 18073203 > | Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova‐Repic A, Stockinger H: Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. JImmunol. 2016 Sep 15; 197(6):2229‐38. < PMID: 27534550 >

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