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CyFlow™ CD203c APC

CyFlow™ CD203c APC
Alternative Name: B10, E-NPP3 , NPP3, PD-1b, PDNP3
Antibody: Yes
Antigen: CD203c
Application: Flow cytometry
Clonality: monoclonal
Clone: NP4D6
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AQ234700

For Research Use Only

$255.00 USD*

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Description
HLDA Workshop HLDA VIII Quantity 100 tests Volume 1.0 mL Immunogen HEK-293 cells... more
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CyFlow™ CD203c APC

HLDA WorkshopHLDA VIII
Quantity100 tests
Volume1.0 mL
ImmunogenHEK-293 cells transfected with human CD203c
Background InformationCD203c (ENPP-3) is integral membrane ectoenzyme (ectonucleotide pyrophosphatase/phosphodiesterase 3), that hydrolyses nucleotide triphosphates and thus modulates purinergic signaling. CD203c is expressed mainly on activated basophils and mast cells. CD203c is upregulated in response to IgE-receptor cross-linking and is overexpressed on neoplastic mast cells in patients with systemic mastocytosis. Measurement of its induced enhancement on the plasma membrane is useful for diagnostics of allergies.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Bühring HJ, Simmons PJ, Pudney M, Müller R, Jarrossay D, van Agthoven A, Willheim M, Brugger W, Valent P, Kanz L: The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood. 1999 Oct 1; 94(7):2343‑56. < PMID: 10498606 > | Bühring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K: The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 31. Blood. 2001 May 15; 97(10):3303‑5. < PMID: 11342463 > | Platz IJ, Binder M, Marxer A, Lischka G, Valent P, Bühring HJ: Hymenoptera‑venom‑induced upregulation of the basophil activation marker ecto‑nucleotide pyrophosphatase/phosphodiesterase 3 in sensitized individuals. Int Arch Allergy Immunol. 2001 Dec; 126(4):335‑42. < PMID: 11815741 > | Hauswirth AW, Escribano L, Prados A, Nuñez R, Mirkina I, Kneidinger M, Florian S, Sonneck K, Vales A, Schernthaner GH, Sanchez-Muñoz L, Sperr WR, Bühring HJ, Orfao A, Valent P: CD203c is overexpressed on neoplastic mast cells in systemic mastocytosis and is upregulated upon IgE receptor cross‑linking. Int J Immunopathol Pharmacol. 2008 Oct-Dec; 21(4):797‑806. < PMID: 19144265 > | Wanich N, Nowak-Wegrzyn A, Sampson HA, Shreffler WG: Allergen‑specific basophil suppression associated with clinical tolerance i patients with milk allergy. J Allergy Clin Immunol. 2009 Apr; 123(4):789‑94e20. < PMID: 19348919 > | Tokuda R, Nagao M, Hiraguchi Y, Hosoki K, Matsuda T, Kouno K, Morita E, Fujisawa T: Antigen‑induced expression of CD203c on basophils predicts IgE‑mediated wheat allergy. Allergol Int. 2009 Jun; 58(2):193‑9. < PMID: 19240377 > | Chirumbolo S, Brizzi M, Ortolani R, Vella A, Bellavite P: Inhibition of CD203c membrane up‑regulation in human basophils by high dilution of histamine: a controlled replication study. Inflamm Res. 2009 Nov; 58(11):755‑64. < PMID: 19418203 >

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