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CyFlow™ CD177 Pacific Blue™

CyFlow™ CD177 Pacific Blue™
Alternative Name: HNA-2a, HNA2A, NB-1 , NB1, PRV1
Antibody: Yes
Antigen: CD177
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-166
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Pacific Blue™
Isotype: IgG1
Laser: Violet
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: BR642789

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Human granulocytes Background... more
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CyFlow™ CD177 Pacific Blue™
Quantity100 tests
Volume0.4 mL
ImmunogenHuman granulocytes
Background InformationCD177 (NB1, HNA-2a and PRV-1 form) is a GPI-anchored glycoprotein present mainly on neutrophils. Its plasma membrane expression is increased during pregnancy and and inflammation or after G-CSF application. Ligand of CD177 has been identified as CD31 (PECAM-1). CD177 participates in neutrophil transmigration and seems to be also a pro-proliferative molecule. The antibodies against CD177 can be involved in neonatal alloimmune neutropenia (NAN).
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Mason D, Simmons D, Buckley C, Schwartz-Albiez R, Hadam M, Saalmuller A, Clark E, Malavasi F, Morrissey JA (Eds): Leucocyte Typing VII. Oxford University Press. 2002; 1‑945. < NLM ID: 101177131 > | Stroncek DF, Caruccio L, Bettinotti M: CD177: A member of the Ly‑6 gene superfamily involved with neutrophil proliferation and polycythemia vera. J Transl Med. 2004 Mar 29; 2(1):8. < PMID: 15050027 > | Mnjoyan Z, Li J, Afshar-Kharghan V: Expression of polycythemia rubra vera‑1 decreases the dependency of cells on growth factors for proliferation. Haematologica. 2005 Mar; 90(3):405‑6. < PMID: 15749675 > | Bauer S, Abdgawad M, Gunnarsson L, Segelmark M, Tapper H, Hellmark T: Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils. J Leukoc Biol. 2007 Feb; 81(2):458‑64. < PMID: 17077162 > | Sachs UJ, Andrei-Selmer CL, Maniar A, Weiss T, Paddock C, Orlova VV, Choi EY, Newman PJ, Preissner KT, Chavakis T, Santoso S: The neutrophil‑specific antigen CD177 is a counter‑receptor for platelet endothelial cell adhesion molecule‑1 (CD31). J Biol Chem. 2007 Aug 10; 282(32):23603‑12. < PMID: 17580308 >

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