CyFlow™ CD163 APC
| Alternative Name: | D11, GH1/61 , M130, RM3/1 |
| Antibody: | Yes |
| Antigen: | CD163 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | GHI/61 |
| Emission Maximum: | 660 nm |
| Excitation Maximum: | 650 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | APC |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | AH476333 |
For Research Use Only
| HLDA Workshop | HLDA VI—WS Code M38 |
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Hairy cell leukemia cells |
| Background Information | CD163 (M130) is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY: A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber‑MAC3, Ki‑M8 and SM4. Immunology. 1992 Apr; 75(4):588‑95. < PMID: 1592433 > | Law SK, Micklem KJ, Shaw JM, Zhang XP, Dong Y, Willis AC, Mason DY: A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. Eur J Immunol. 1993 Sep; 23(9):2320‑5. < PMID: 8370408 > | Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Møller HJ, Peterslund NA, Graversen JH, Moestrup SK: Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma. Blood. 2002 Jan 1; 99(1):378‑80. < PMID: 11756196 > | Philippidis P, Mason JC, Evans BJ, Nadra I, Taylor KM, Haskard DO, Landis RC: Hemoglobin scavenger receptor CD163 mediates interleukin‑10 release and heme oxygenase‑1 synthesis: antiinflammatory monocyte‑macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery. Circ Res. 2004 Jan 9; 94(1):119‑26. < PMID: 14656926 > | Madsen M, Møller HJ, Nielsen MJ, Jacobsen C, Graversen JH, van den Berg T, Moestrup SK: Molecular characterization of the haptoglobin‑hemoglobin receptor CD163: Ligand binding properties of the scavenger receptor cysteine‑rich domain region. J Biol Chem. 2004 Dec 3; 279(49):51561‑7. < PMID: 15448162 > | Kim WK, Alvarez X, Fisher J, Bronfin B, Westmoreland S, McLaurin J, Williams K: CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood. Am J Pathol. 2006 Mar; 168(3):822‑34. < PMID: 16507898 > | Moniuszko M, Kowal K, Rusak M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin Vaccine Immunol. 2006 Jun; 13(6):704‑7. < PMID: 16760331 > | Bover LC, Cardó-Vila M, Kuniyasu A, Sun J, Rangel R, Takeya M, Aggarwal BB, Arap W, Pasqualini R: A previously unrecognized protein‑protein interaction between TWEAK and CD163: potential biological implications. J Immunol. 2007 Jun 15; 178(12):8183‑94. < PMID: 17548657 > | Kusi KA, Gyan BA, Goka BQ, Dodoo D, Obeng-Adjei G, Troye-Blomberg M, Akanmori BD, Adjimani JP: Levels of soluble CD163 and severity of malaria in children in Ghana. Clin Vaccine Immunol. 2008 Sep; 15(9):1456‑60. < PMID: 18632918 >