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CyFlow™ CD162 APC

CyFlow™ CD162 APC
Alternative Name: PSGL-1, PSGL1
Antibody: Yes
Antigen: CD162
Application: Flow cytometry
Clonality: monoclonal
Clone: TC2
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CY848695

For Research Use Only

$255.00 USD*

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 1.0 mL Immunogen Human thymocytes Background Information... more
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CyFlow™ CD162 APC
Quantity100 tests
Volume1.0 mL
ImmunogenHuman thymocytes
Background InformationCD162 ( PSGL-1; P-selectin glycoprotein ligand-1) is a sialomucin constitutively expressed as a disulfide-linked homodimer of two 120 kDa subunits on the surface of circulating leukocytes. CD162 serves as a ligand for P- E- and L-selectin, with the highest affinity for P-selectin. It is thus involved in leukocyte rolling at the endothelial surfaces, prerequisite for firm leukocyte adhesion and subsequent transendothelial migration. CD162 also mediates leukocyte-platelet adhesion and interleukocyte contacts. Whereas serving as an adhession molecule on mature leukocytes, CD162 is a potent negative regulator of human hematopoietic progenitors.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Moore KL: Structure and function of P‑selectin glycoprotein ligand‑1. Leuk Lymphoma. 1998 Mar; 29(1‑2):42005. < PMID: 9638971 > | Lévesque JP, Zannettino AC, Pudney M, Niutta S, Haylock DN, Snapp KR, Kansas GS, Berndt MC, Simmons PJ: PSGL‑1‑mediated adhesion of human hematopoietic progenitors to P‑selectin results in suppression of hematopoiesis. Immunity. 1999 Sep; 11(3):369‑78. < PMID: 10514015 > | Davenpeck KL, Brummet ME, Hudson SA, Mayer RJ, Bochner BS: Activation of human leukocyte reduces surface P‑selectin glycoprotein ligand‑1 (PSGL‑1, CD162 and adhesion to P‑selectin in vitro. J Immunol. 2000 Sep 1; 165(5):2764‑72. < PMID: 10946308 > | Mason D, Simmons D, Buckley C, Schwartz-Albiez R, Hadam M, Saalmuller A, Clark E, Malavasi F, Morrissey JA (Eds): Leucocyte Typing VII. Oxford University Press. 2002; 1‑945. < NLM ID: 101177131 > | Marsik C, Mayr F, Cardona F, Schaller G, Wagner OF, Jilma B: Endotoxin down‑modulates P‑selectin glycoprotein ligand‑1 (PSGL‑1, CD162) on neutrophils in humans. J Clin Immunol. 2004 Jan; 24(1):62‑5. < PMID: 14997035 >

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