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CyFlow™ CD158d APC

CyFlow™ CD158d APC
Alternative Name: 15.212, 103AS, KIR2DL4 , KIR103, KIR103AS
Antibody: Yes
Antigen: CD158d
Application: Flow cytometry
Clonality: monoclonal
Clone: mAb#33
Emission Maximum: 660 nm
Excitation Maximum: 650 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: APC
Isotype: IgG1
Laser: Red
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BN814356

For Research Use Only

$255.00 USD*

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 1.0 mL Immunogen NK3.3 cells and KIR2DL4-Ig fusion protein... more
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CyFlow™ CD158d APC
Quantity100 tests
Volume1.0 mL
ImmunogenNK3.3 cells and KIR2DL4-Ig fusion protein
Background InformationCD158d (KIR2DL4) is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Rajagopalan S, Fu J, Long EO: Cutting edge: induction of IFN‑gamma production but not cytotoxicity by the killer cell Ig‑like receptor KIR2DL4 (CD158d) in resting NK cells. J Immunol. 2001 Aug 15; 167(4):1877‑81. < PMID: 11489965 > | Goodridge JP, Witt CS, Christiansen FT, Warren HS: KIR2DL4 (CD158d) genotype influences expression and function in NK cells. J Immunol. 2003 Aug 15; 171(4):1768‑74. < PMID: 12902476 > | Yan WH, Fan LA: Residues Met76 and Gln79 in HLA‑G alpha1 domain involve in KIR2DL4 recognition. Cell Res. 2005 Mar; 15(3):176‑82. < PMID: 15780179 > | LeMaoult J, Zafaranloo K, Le Danff C, Carosella ED: HLA‑G up‑regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells. FASEB J. 2005 Apr; 19(6):662‑4. < PMID: 15670976 > | Rajagopalan S, Bryceson YT, Kuppusamy SP, Geraghty DE, van der Meer A, Joosten I, Long EO: Activation of NK cells by an endocytosed receptor for soluble HLA‑G. PLoS Biol. 2006 Jan; 4(1):e9. < PMID: 16366734 >

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