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CyFlow™ CD154 PE

CyFlow™ CD154 PE
Alternative Name: CD40L , gp39, IMD3, T-BAM, TNFSF5, TRAP-1
Antibody: Yes
Antigen: CD154
Application: Flow cytometry
Clonality: monoclonal
Clone: 24-31
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Isotype: IgG1
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Non-Human Primates
Product number: AG649313

For Research Use Only

$255.00 USD*

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 1.0 mL Immunogen Human CD154 fusion protein Background... more
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CyFlow™ CD154 PE
Quantity100 tests
Volume1.0 mL
ImmunogenHuman CD154 fusion protein
Background InformationCD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kornbluth RS, Kee K, Richman DD: CD40 ligand (CD154) stimulation of macrophages to produce HIV‑1‑suppressive beta‑chemokines. Proc Natl Acad Sci USA. 1998 Apr 28; 95(9):5205‑10. < PMID: 9560254 > | Grammer AC, McFarland RD, Heaney J, Darnell BF, Lipsky PE: Expression, regulation, and function of B cell‑expressed CD154 in germinal centers. J Immunol. 1999 Oct 15; 163(8):4150‑9. < PMID: 10510350 > | Barnhart B, Ford GS, Bhushan A, Song C, Covey LR: A polymorphic CD40 ligand (CD154) molecule mediates CD40‑dependent signalling but interferes with the ability of soluble CD40 to functionally block CD154:CD40 interactions. Immunology. 2000 Jan; 99(1):54‑61. < PMID: 10651941 > | Berner B, Wolf G, Hummel KM, Müller GA, Reuss-Borst MA: Increased expression of CD40 ligand (CD154) on CD4+ T cells as a marker of disease activity in rheumatoid arthritis. Ann Rheum Dis. 2000 Mar; 59(3):190‑5. < PMID: 10700427 > | Brams P, Black A, Padlan EA, Hariharan K, Leonard J, Chambers-Slater K, Noelle RJ, Newman R: A humanized anti‑human CD154 monoclonal antibody blocks CD154‑CD40 mediated human B cell activation. Int Immunopharmacol. 2001 Feb; 1(2):277‑94. < PMID: 11360929 > | Kum WW, Hung RW, Cameron SB, Chow AW: Temporal sequence and functional implications of V beta‑specific T cell receptor down‑regulation and costimulatory molecule expression following in vitro stimulation with the staphylococcal superantigen Toxic shock syndrome toxin‑1. J Infect Dis. 2002 Feb 15; 185(4):555‑60. < PMID: 11865410 > | Fernandes MS, Gomes EM, Butcher LD, Hernandez-Alcoceba R, Chang D, Kansopon J, Newman J, Stone MJ, Tong AW: Growth inhibition of human multiple myeloma cells by an oncolytic adenovirus carrying the CD40 ligand transgene. Clin Cancer Res. 2009 Aug 1; 15(15):4847‑56. < PMID: 19622582 >

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