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CyFlow™ CD148 Biotin

CyFlow™ CD148 Biotin
Alternative Name: DEP-1 , HPTP-a, SCC1
Antibody: Yes
Antigen: CD148
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-CD148/05
Field of Interest: Immunophenotyping, Phosphorylation
Format/Fluorochrome: Biotin
Isotype: IgG2b
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: AE191755

For Research Use Only

$160.00 USD*

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Description
Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
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CyFlow™ CD148 Biotin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenHuman recombinant CD148 (amino acids 1-444)
Background InformationCD148 (HPTP-eta, DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leukocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Stepanek O, Kalina T, Draber P, Skopcova T, Svojgr K, Angelisova P, Horejsi V, Weiss A, Brdicka T: Regulation of Src family kinases involved in T cell receptor signaling by protein‑tyrosine phosphatase CD148. J Biol Chem. 2011 Jun 24; 286(25):22101‑12. < PMID: 21543337 >

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