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CyFlow™ CD148 PE

CyFlow™ CD148 PE
Alternative Name: DEP-1 , HPTP-a, SCC1
Antibody: Yes
Antigen: CD148
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-CD148/05
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping, Phosphorylation
Format/Fluorochrome: PE
Isotype: IgG2b
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: BH775758

For Research Use Only

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Description Documentation TDS & SDS
Description
Quantity 100 tests Volume 1.0 mL Immunogen Human recombinant CD148 (amino acids... more
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CyFlow™ CD148 PE
Quantity100 tests
Volume1.0 mL
ImmunogenHuman recombinant CD148 (amino acids 1-444)
Background InformationCD148 (HPTP-eta, DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leukocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Stepanek O, Kalina T, Draber P, Skopcova T, Svojgr K, Angelisova P, Horejsi V, Weiss A, Brdicka T: Regulation of Src family kinases involved in T cell receptor signaling by protein‑tyrosine phosphatase CD148. J Biol Chem. 2011 Jun 24; 286(25):22101‑12. < PMID: 21543337 >

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