CyFlow™ CD147 FITC
| Alternative Name: | 5F7, Basigin , BSG, EMMPRIN, M6, TCSF |
| Antibody: | Yes |
| Antigen: | CD147 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | MEM-M6/1 |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | FITC |
| Isotype: | IgG1 |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | BG442445 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 2.0 mL |
| Immunogen | Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer) |
| Background Information | CD147 (basigin, neurothelin, OX-47, 5A11, CE9, M6) also known as EMMPRIN (extracellular matrix metalloproteinase inducer) or TCSF (tumour cell-derived collagenase-stimulatory factor) is an ubiquitously expressed cell surface protein with multiple glycosylated forms. The highest level of CD147 expression is on metabolically active cells, such as lymphoblasts, inflammatory cells, brown adipocytes and malignant tumor cells. CD147 has multiple functions, including facilitating of cell surface expression of monocarboxylate transporter proteins and extracellular matrix metalloproteinases, regulation of integrin functions, it plays roles in cell development and activation, fetal development or retinal function. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Koch C, Staffler G, Huttinger R, Hilgert I, Prager E, Cerny J, Steinlein P, Majdic O, Horejsi V, Stockinger H: T cell activation‑associated epitopes of CD147 in regulation of the T cell response, and their definition by antibody affinity and antigen density. Int Immunol. 1999 May; 11(5):777‑86. < PMID: 10330283 > | Kirk P, Wilson MC, Heddle C, Brown MH, Barclay AN, Halestrap AP: CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression. EMBO J. 2000 Aug 1; 19(15):3896‑904. < PMID: 10921872 > | Wilson MC, Meredith D, Fox JE, Manoharan C, Davies AJ, Halestrap AP: Basigin (CD147 is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 2005 Jul 22; 280(29):27213‑21. < PMID: 15917240 > | Xu D, Hemler ME: Metabolic activation‑related CD147‑CD98 complex. Mol Cell Proteomics. 2005 Aug; 4(8):1061‑71. < PMID: 15901826 > | Iacono KT, Brown AL, Greene MI, Saouaf SJ: CD147 immunoglobulin superfamily receptor function and role in pathology. Exp Mol Pathol. 2007 Dec; 83(3):283‑95. < PMID: 17945211 > | Melchior A, Denys A, Deligny A, Mazurier J, Allain F: Cyclophilin B induces integrin‑mediated cell adhesion by a mechanism involving CD98‑dependent activation of protein kinase C‑delta and p44/42 mitogen‑activated protein kinases. Exp Cell Res. 2008 Feb 1; 314(3):616‑28. < PMID: 18054915 > | Schmidt R, Bültmann A, Fischel S, Gillitzer A, Cullen P, Walch A, Jost P, Ungerer M, Tolley ND, Lindemann S, Gawaz M, Schömig A, May AE: Extracellular matrix metalloproteinase inducer (CD147 is a novel receptor on platelets, activates platelets, and augments nuclear factor kappaB‑dependent inflammation in monocytes. Circ Res. 2008 Feb 15; 102(3):302‑9. < PMID: 18048771 > | Ruiz S, Castro-Castro A, Bustelo XR: CD147 Inhibits the Nuclear Factor of Activated T‑cells by Impairing Vav1 and Rac1 Downstream Signaling. J Biol Chem. 2008 Feb 29; 283(9):5554‑66. < PMID: 18160397 > | Ohradanova‐Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco‐Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. FrontImmunol. 2018 Apr 27; 9:852. < PMID: 29780382 >