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CyFlow™ CD117 PE-Cy5

CyFlow™ CD117 PE-Cy5
Alternative Name: c-kit , SCFR
Antibody: Yes
Antigen: CD117
Application: Flow cytometry
Clonality: monoclonal
Clone: 104D2
Emission Maximum: 670 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping, Phosphorylation
Format/Fluorochrome: PE-Cy5
Isotype: IgG1
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Cow, Human, Non-Human Primates
Product number: CH470016

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Description
HLDA Workshop HLDA VI—WS Code C-30 Quantity 100 tests Volume 0.4 mL Immunogen... more
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CyFlow™ CD117 PE-Cy5
HLDA WorkshopHLDA VI—WS Code C-30
Quantity100 tests
Volume0.4 mL
ImmunogenMOLM-1 megakaryocytic cells
Background InformationCD117 (c-Kit) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 can lead to growth and progression of tumors. After binding of its ligand, SCF (stem cell factor), CD117 is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland Publishing Inc, New York. 1997; 1‑1342. < NLM ID: 9712219 > | Rappold I, Ziegler BL, Köhler I, Marchetto S, Rosnet O, Birnbaum D, Simmons PJ, Zannettino AC, Hill B, Neu S, Knapp W, Alitalo R, Alitalo K, Ullrich A, Kanz L, Bühring HJ: Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase. Blood. 1997 Jul 1; 90(1):111‑25. < PMID: 9207445 > | Broudy VC, Lin NL, Bühring HJ, Komatsu N, Kavanagh TJ: Analysis of c‑kit receptor dimerization by fluorescence resonance energy transfer. Blood. 1998 Feb 1; 91(3):898‑906. < PMID: 9446650 > | Broudy VC, Lin NL, Liles WC, Corey SJ, O'Laughlin B, Mou S, Linnekin D: Signaling via Src family kinases is required for normal internalization of the receptor c‑Kit. Blood. 1999 Sep 15; 94(6):1979‑86. < PMID: 10477727 > | Yoshino N, Ami Y, Terao K, Tashiro F, Honda M: Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti‑human cross‑reactive antibodies. Exp Anim. 2000 Apr; 49(2):97‑110. < PMID: 10889948 > | Blair A, Sutherland HJ: Primitive acute myeloid leukemia cells with long‑term proliferative ability in vitro and in vivo lack surface expression of c‑kit (CD117). Exp Hematol. 2000 Jun; 28(6):660‑71. < PMID: 10880752 > | Wihlidal P, Varga F, Pfeilstöcker M, Karlic H: Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies. Leuk Res. 2006 Oct; 30(10):1241‑8. < PMID: 16387359 > | Nagano M, Yamashita T, Hamada H, Ohneda K, Kimura K, Nakagawa T, Shibuya M, Yoshikawa H, Ohneda O: Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood. 2007 Jul 1; 110(1):151‑60. < PMID: 17379743 > | Stevenson KS, McGlynn L, Hodge M, McLinden H, George WD, Davies RW, Shiels PG: Isolation, Characterisation and Differentiation of Thy1.1 sorted Pancreatic Adult Progenitor Cell Populations. Stem Cells Dev. 2009 Dec; 18(10):1389‑98. < PMID: 19326970 >

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