CyFlow™ CD107b APC
| Alternative Name: | LAMP-2 |
| Antibody: | Yes |
| Antigen: | CD107b |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | H4B4 |
| Emission Maximum: | 660 nm |
| Excitation Maximum: | 650 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | APC |
| Isotype: | IgG1 |
| Laser: | Red |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CT778572 |
For Research Use Only
| Quantity | 100 tests |
| Volume | 1.0 mL |
| Immunogen | Human peripheral blood mononuclear cells (PBMC) |
| Background Information | CD107b (LAMP-2; lysosome-associated membrane protein-2), together with CD107a (LAMP-1), is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, especially CD107b is important regulator in successful phagosomal maturation. CD107b deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danons disease). Immature CD107b is an approximately 45 kDa protein, but after extensive glycosylation the mature glycoprotein has about 100-120 kDa. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.02% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
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