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CyFlow™ CD105 Alexa Fluor™ 488

CyFlow™ CD105 Alexa Fluor™ 488
Alternative Name: Endoglin
Antibody: Yes
Antigen: CD105
Application: Flow cytometry
Clonality: monoclonal
Clone: MEM-226
Emission Maximum: 519 nm
Excitation Maximum: 495 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: Alexa Fluor™ 488
Isotype: IgG2a
Laser: Blue
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human, Rat
Product number: BM597424

For Research Use Only

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Description
Quantity 100 tests Volume 0.4 mL Immunogen Recombinant Vaccinia virus containing the... more
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CyFlow™ CD105 Alexa Fluor™ 488
Quantity100 tests
Volume0.4 mL
ImmunogenRecombinant Vaccinia virus containing the human CD105 cDNA
Background InformationCD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFβR-2 as a receptor for TGFβ-1 and TGFβ-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonizes the inhibitory effects of TGFβ-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Li C, Issa R, Kumar P, Hampson IN, Lopez-Novoa JM, Bernabeu C, Kumar S: CD105 prevents apoptosis in hypoxic endothelial cells. J Cell Sci. 2003 Jul 1; 116(13):2677‑85. < PMID: 12746487 > | Zhu Y, Sun Y, Xie L, Jin K, Sheibani N, Greenberg DA: Hypoxic induction of endoglin via mitogen‑activated protein kinases in mouse brain microvascular endothelial cells. Stroke. 2003 Oct; 34(10):2483‑8. < PMID: 12947156 > | Guo B, Slevin M, Li C, Parameshwar S, Liu D, Kumar P, Bernabeu C, Kumar S: CD105 inhibits transforming growth factor‑beta‑Smad3 signalling. Anticancer Res. 2004 May-Jun; 24(3a):1337‑45. < PMID: 15274293 > | Warrington K, Hillarby MC, Li C, Letarte M, Kumar S: Functional role of CD105 in TGF‑beta1 signalling in murine and human endothelial cells. Anticancer Res. 2005 May-Jun; 25(3B):1851‑64. < PMID: 16158917 > | Piao M, Tokunaga O: Significant expression of endoglin (CD105), TGFbeta‑1 and TGFbeta R‑2 in the atherosclerotic aorta: an immunohistological study. J Atheroscler Thromb. 2006 Apr; 13(2):82‑9. < PMID: 16733295 > | Schmidt D, Achermann J, Odermatt B, Breymann C, Mol A, Genoni M, Zund G, Hoerstrup SP: Prenatally fabricated autologous human living heart valves based on amniotic fluid derived progenitor cells as single cell source. Circulation. 2007 Sep 11; 116(11 Suppl):I64‑70. < PMID: 17846327 > | Asumda FZ, Chase PB: Age‐related changes in rat bone‐marrow mesenchymal stem cell plasticity. BMCCellBiol. 2011 Oct 12; 12:44. < PMID: 21992089 >

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