CyFlow™ CD94 FITC
| Alternative Name: | Kp43 |
| Antibody: | Yes |
| Antigen: | CD94 |
| Application: | Flow cytometry |
| Clonality: | monoclonal |
| Clone: | HP-3D9 |
| Emission Maximum: | 518 nm |
| Excitation Maximum: | 490 to 495 nm |
| Field of Interest: | Immunophenotyping |
| Format/Fluorochrome: | FITC |
| Isotype: | IgG1 |
| Laser: | Blue |
| Regulatory Status: | RUO |
| Source Species: | Mouse |
| Target Species: | Human |
| Product number: | CN818854 |
For Research Use Only
| HLDA Workshop | HLDA V—WS Code NK82 |
| Quantity | 100 tests |
| Volume | 0.4 mL |
| Immunogen | Cultured human NK cells |
| Background Information | CD94 (KLRD1; killer cell lectin-like receptor D1) is a transmembrane glycoprotein of the C-type lectin family, which forms disulfide-linked heterodimers with NKG2A, B, C, E, H proteins, constituting functionally distinct receptors of NK cells and related cell types. CD94/NKG2A and CD94/NKG2B heterodimers serve as inhibitory, whereas CD94/NKG2C and CD94/NKG2E as activating receptors. The ligand for CD94/NKG2 complexes has been identified as HLA-E. Extent of CD94 expression on NK cell surface can be used to demonstrate their progress through the differentiation process. |
| Usage | The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. |
| Storage Buffer | The reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide. |
| Storage | Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze. |
| Stability | Do not use after expiration date stamped on vial label. |
| Phillips JH, Chang C, Mattson J, Gumperz JE, Parham P, Lanier LL: CD94 and a novel associated protein (94AP) form a NK cell receptor involved in the recognition of HLA‑A, HLA‑B, and HLA‑C allotypes. Immunity. 1996 Aug; 5(2):163‑72. < PMID: 8769479 > | Seo N, Tokura Y, Ishihara S, Takeoka Y, Tagawa S, Takigawa M: Disordered expression of inhibitory receptors on the NK1‑type natural killer (NK) leukaemic cells from patients with hypersensitivity to mosquito bites. Clin Exp Immunol. 2000 Jun; 120(3):413‑9. < PMID: 10844517 > | Ntrivalas EI, Kwak-Kim JY, Gilman-Sachs A, Chung-Bang H, Ng SC, Beaman KD, Mantouvalos HP, Beer AE: Status of peripheral blood natural killer cells in women with recurrent spontaneous abortions and infertility of unknown aetiology. Hum Reprod. 2001 May; 16(5):855‑61. < PMID: 11331628 > | Romero P, Ortega C, Palma A, Molina IJ, Peña J, Santamaría M: Expression of CD94 and NKG2 molecules on human CD4(+) T cells in response to CD3‑mediated stimulation. J Leukoc Biol. 2001 Aug; 70(2):219‑24. < PMID: 11493613 > | Wada H, Matsumoto N, Maenaka K, Suzuki K, Yamamoto K: The inhibitory NK cell receptor CD94/NKG2A and the activating receptor CD94/NKG2C bind the top of HLA‑E through mostly shared but partly distinct sets of HLA‑E residues. Eur J Immunol. 2004 Jan; 34(1):81‑90. < PMID: 14971033 > | Bovenschen HJ, Van De Kerkhof PC, Gerritsen WJ, Seyger MM: The role of lesional T cells in recalcitrant psoriasis during infliximab therapy. Eur J Dermatol. 2005 Nov-Dec; 15(6):454‑8. < PMID: 16280298 > | Hallermalm K, Seki K, De Geer A, Motyka B, Bleackley RC, Jager MJ, Froelich CJ, Kiessling R, Levitsky V, Levitskaya J: Modulation of the tumor cell phenotype by IFN‑gamma results in resistance of uveal melanoma cells to granule‑mediated lysis by cytotoxic lymphocytes. J Immunol. 2008 Mar 15; 180(6):3766‑74. < PMID: 18322182 > | Yu J, Mao HC, Wei M, Hughes T, Zhang J, Park IK, Liu S, McClory S, Marcucci G, Trotta R, Caligiuri MA: CD94 surface density identifies a functional intermediary between the CD56bright and CD56dim human NK‑cell subsets. Blood. 2010 Jan 14; 115(2):274‑81. < PMID: 19897577 >