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CyFlow™ CD82 PE

CyFlow™ CD82 PE
Alternative Name: C33, R2l 4F9
Antibody: Yes
Antigen: CD82
Application: Flow cytometry
Clonality: monoclonal
Clone: C33
Emission Maximum: 576 nm
Excitation Maximum: 496 nm, 565 nm
Field of Interest: Immunophenotyping
Format/Fluorochrome: PE
Isotype: IgG2a
Laser: Blue , Green, Yellow
Regulatory Status: RUO
Source Species: Mouse
Target Species: Human
Product number: CP903161

For Research Use Only

$255.00 USD*

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Description
Quantity 100 tests Volume 1.0 mL Immunogen C91/PL (human HTLV-1+ T cell line)... more
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CyFlow™ CD82 PE
Quantity100 tests
Volume1.0 mL
ImmunogenC91/PL (human HTLV-1+ T cell line)
Background InformationCD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumor-suppressive roles of CD82 have been demonstrated.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
Specific References

| Fukudome K, Furuse M, Imai T, Nishimura M, Takagi S, Hinuma Y, Yoshie O: Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T‑cell leukemia virus type 1 (HTLV‑1)‑induced syncytium formation: altered glycosylation of C33 antigen in HTLV‑1‑positive T cells. J Virol. 1992 Mar; 66(3):1394‑401. < PMID: 1738199 > | Imai T, Yoshie O: C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and associate with each other and with CD4 or CD8 in T cells. J Immunol. 1993 Dec 1; 151(11):6470‑81. < PMID: 8245480 > | Imai T, Kakizaki M, Nishimura M, Yoshie O: Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82. J Immunol. 1995 Aug 1; 155(3):1229‑39. < PMID: 7636191 > | Ueda T, Ichikawa T, Tamaru J, Mikata A, Akakura K, Akimoto S, Imai T, Yoshie O, Shiraishi T, Yatani R, Ito H, Shimazaki J: Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer. Am J Pathol. 1996 Nov; 149(5):1435‑40. < PMID: 8909232 > | Escola JM, Kleijmeer MJ, Stoorvogel W, Griffith JM, Yoshie O, Geuze HJ: Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B‑lymphocytes. J Biol Chem. 1998 Aug 7; 273(32):20121‑7. < PMID: 9685355 >

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